Complete genome sequences and phylogenetic analysis of hepatitis delta viruses isolated from nine Turkish patients
Complete genome sequences and phylogenetic analysis of hepatitis delta viruses isolated from nine Turkish patients
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DOI：
10.1007/s00705-011-1120-y
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发表时间：
2011-12-01
影响因子：
2.7
通讯作者：
Bozdayi, A. Mithat
中科院分区：
文献类型：
--
作者：
Celik, Inci;Karatayli, Ersin;Bozdayi, A. Mithat
丁型肝炎病毒(HDV)是乙肝病毒(乙肝病毒)的亚病毒病原体，其生命周期依赖于乙肝病毒。一般认为，HDV有8种不同的基因型别。在这项研究中，使用两对覆盖整个HDV基因组的引物，通过RT-PCR获得了从9名土耳其患者中分离的HDV基因组的完整核苷酸序列。对扩增产物直接测序。结果表明，这9株毒株全长约1680个碱基对，与GenBank中检索到的代表各型的HDV基因组全序列一起进行系统发育分析，均聚在HDV-1基因分支上。对丁型肝炎病毒L部分基因序列的分析表明，土耳其株与HDV-1的序列相似性最高(90.5%)，而与HDV-3的序列相似性最低(64.1%)，序列相似性在87.4-97.1%之间。全基因组分析表明，土耳其分离株与HDV-1的序列相似性在80.7%~95.4%之间，最高相似性为84.8%。土耳其株与HDV-3的序列相似性最低为56.4%。综上所述，系统发育分析表明，土耳其的HDV分离株属于HDV-1。
Hepatitis delta virus (HDV) is a subviral agent of hepatitis B virus (HBV), and its life cycle is dependent on HBV. It is commonly accepted that HDV has eight distinct genotypes. In this study, the complete nucleotide sequences of HDV genomes isolated from nine Turkish patients were obtained by RT-PCR using two pairs of primers that cover the entire HDV genome. PCR products were sequenced directly. The results showed that these 9 isolates were approximately 1680 base pairs in length and clustered in the genotype HDV-1 branch when phylogenetic analysis was done with the sequences together with the complete sequences of HDV genomes representing each genotype retrieved from GenBank. Analysis of a portion of the large hepatitis D antigen (L-HDAg) gene showed that sequence similarity among these Turkish isolates is between 87.4 and 97.1%, and the Turkish isolates have the most sequence similarity to HDV-1 (90.5%), while they have the least sequence similarity to HDV-3 (64.1%). Full-genome analysis indicates that the sequence similarity is between 80.7 and 95.4%, and the highest sequence similarity is 84.8% (between the Turkish isolates and HDV-1). The lowest sequence similarity is 56.4% (between the Turkish isolates and HDV-3). In conclusion, phylogenetic analysis shows that the Turkish HDV isolates belong to HDV-1.