Reductions in Urinary Collection Frequency for Assessment of Reproductive Hormones Provide Physiologically Representative Exposure and Mean Concentrations when Compared with Daily Collection
Reductions in Urinary Collection Frequency for Assessment of Reproductive Hormones Provide Physiologically Representative Exposure and Mean Concentrations when Compared with Daily Collection
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DOI：
10.1002/ajhb.22649
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发表时间：
2015-05-01
影响因子：
2.9
通讯作者：
De Souza, Mary Jane
中科院分区：
文献类型：
--
作者：
Allaway, Heather C. M.;Williams, Nancy I.;De Souza, Mary Jane
目的确定在月经周期或28天闭经监测期间，将尿样采集频率从每天减少到每周5、3或2天，是否能准确反映生殖激素代谢物雌激素-1-葡萄糖醛酸酯（E1G）和妊娠二醇葡萄糖醛酸酯（PdG）的暴露和平均浓度。方法出现痛经或运动相关月经紊乱的运动女性每天采集尿液样本，评估E1G和PdG浓度。在对每日样本进行酶免疫分析后，系统地从每个月经周期或闭经监测期间删除E1G和PdG数据，以模拟三种减少的收集频率，分别代表每周5天、3天和2天。计算激素和所有四种尿液收集频率的暴露量和平均浓度。结果减少采集频率得到的g和PdG暴露量和平均周期浓度与每日采集频率无显著差异（P>0.05），与月经周期和监测周期是否单独分析无关。Bland-Altman分析表明，每次减少的收集频率与每日收集之间存在可接受的一致性。结论与每日收集尿液相比，每周减少5天、3天或2天的收集次数可以准确地反映不同长度和类型的月经功能收集期的E1G和PdG。减少尿液样本采集频率可以使研究人员减轻参与者的负担和成本，提高依从性，并研究更广泛的研究人群。点。j .的嗡嗡声。《圣经》27:35 -371,2015。(c) 2014 Wiley期刊公司
ObjectiveTo determine if reducing the frequency of urinary sample collection from daily to 5, 3, or 2 days per week during a menstrual cycle or 28-day amenorrheic monitoring period provide accurate representations of the reproductive hormone metabolites estrone-1-glucuronide (E1G) and pregnanediol glucuronide (PdG) exposure and mean concentrations.MethodsExercising women presenting with eumenorrhea or exercise-associated menstrual disturbances collected daily urine samples for the assessment of E1G and PdG concentrations. After enzyme immunoassay analysis of the daily samples, E1G and PdG data were systematically removed from each menstrual cycle or amenorrheic monitoring period to mimic three reduced collection frequencies, representing 5, 3, and 2 days per week. Exposure and mean concentration were calculated for both hormones and all four urinary collection frequencies.ResultsE1G and PdG exposure and mean cycle concentrations derived from reduced collection frequencies were not different from daily collection (P>0.05), independent of whether menstrual cycles and monitoring periods were analyzed together or separately. Bland-Altman analysis indicated acceptable agreement between each reduced collection frequency and daily collection.ConclusionsCompared with daily urinary collection, a reduced collection frequency of 5, 3, or 2 days each week provides accurate E1G and PdG profiles of collection periods of various lengths and types of menstrual function. Reduction of urinary sample collection frequency may enable researchers to reduce participant burden and costs, increase compliance, and study a wider range of study populations. Am. J. Hum. Biol. 27:358-371, 2015. (c) 2014 Wiley Periodicals, Inc.