Mutations in p53 produce a common conformational effect that can be detected with a panel of monoclonal antibodies directed toward the central part of the p53 protein.
Mutations in p53 produce a common conformational effect that can be detected with a panel of monoclonal antibodies directed toward the central part of the p53 protein.
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p53 突变产生一种常见的构象效应，可以通过一组针对 p53 蛋白中心部分的单克隆抗体进行检测。
作者：
Y. Legros;A. Meyer;K. Ory;Thierry Soussi
人p53显示位于蛋白质的氨基和羧基末端的两个免疫显性区域。使用截短的p53（残基66至361），我们选择了8个新的单克隆抗体针对蛋白质的中心部分。我们用一组重叠肽鉴定了八种抗体中七种抗体识别的表位。其中一种抗体具有与PAb240相似的表位，而其他抗体识别新的和不同的抗原决定簇。使用一系列的19 p53突变体，我们表明，几个新的单克隆抗体的行为是类似的PAb240，尽管他们的各种表位定位。这表明，p53蛋白中的不同突变诱导了整体构象变化，可以通过针对蛋白质中心部分的各种单克隆抗体检测到这种变化。
Human p53 displays two immunodominant regions localized in the amino and carboxy termini of the protein. Using a truncated p53 (residues 66 to 361), we selected eight new monoclonal antibodies directed to the central part of the protein. We identified the epitopes recognized by seven out of eight antibodies with a set of overlapping peptides. One of these antibodies had an epitope similar to PAb240, whereas the others recognized novel and diverse antigenic determinants. Using a series of 19 p53 mutants, we show that the behavior of several of the new monoclonal antibodies is similar to that of PAb240 despite their various epitope localizations. This suggests that different mutations in the p53 protein induce an overall conformational change that can be detected by various monoclonal antibodies directed toward the central part of the protein.