Comparison of the Odontogenic Differentiation Potential of Dental Follicle, Dental Papilla, and Cranial Neural Crest Cells
Comparison of the Odontogenic Differentiation Potential of Dental Follicle, Dental Papilla, and Cranial Neural Crest Cells
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牙囊、牙乳头和颅神经嵴细胞牙源分化潜能的比较
DOI：
10.1016/j.joen.2015.03.003
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发表时间：
2015-07-01
影响因子：
4.2
通讯作者：
Tian, Weidong
中科院分区：
文献类型：
--
作者：
Chen, Gang;Sun, Qince;Tian, Weidong
简介：在牙齿发育过程中，起源于神经嵴的细胞充当牙囊和牙乳头中细胞的前体。因此，本研究旨在了解颅神经嵴细胞（CNCC），牙囊细胞（DFC），和牙乳头细胞（DPC）的协会进行平行比较，以评估其牙向分化能力。研究方法：本研究从C57/绿色荧光蛋白阳性的小鼠或胚胎中获得3种细胞，比较其细胞形态、表面抗原、显微结构以及基因和蛋白表达。在经处理的牙本质基质提供的牙源性微环境下，进一步比较3种细胞在体内外的牙源性分化情况。结果如下：神经丝蛋白、微管蛋白和巢蛋白的DFC基因表达水平与牙本质前体细胞相近，碱性磷酸酶、骨桥蛋白、牙本质基质蛋白1和牙本质涎磷蛋白的DFC基因表达水平最低。然而，Western blot结果显示，DFC具有更相似的蛋白质谱CNCC比DPC，包括胶原蛋白1，转化生长因子β 1，骨桥蛋白，神经丝，和牙本质基质蛋白1。同时，DFCs作为牙源性干细胞的来源之一，在体外具有良好的牙源性分化能力。此外，在所有3组中观察到相似的牙本质样组织。结论：CNCC、DFC和DPC在牙源性分化中具有不同的生物学特性。
Introduction: During tooth development, cells originating from the neural crest serve as precursors to the cells in the dental follicle and dental papilla. Therefore, the current study aimed to understand the associations of cranial neural crest cells (CNCCs), dental follicle cells (DFCs), and dental papilla cells (DPCs) by performing a parallel comparison to evaluate their odontogenic differentiation capacities. Methods: In this study, we harvested the 3 cells from C57/green fluorescent protein positive mice or embryos and compared the cell morphology, surface antigens, microstructures, and gene and protein expression. Under the odontogenic microenvironments provided by treated dentin matrix, the odontogenic differentiations of the 3 cells were further compared in vitro and in vivo. Results: The gene levels of DFCs in neurofilament, tubulin, and nestin were close to the DPCs, and in alkaline phosphatase, osteopontin, dentin matrix protein 1, and dentin sialophosphoprotein were the lowest in the 3 cells. However, Western blot results showed that DFCs possessed more similar protein profiles to CNCCs than DPCs, including collagen 1, transforming growth factor beta 1, osteopontin, neurofilament, and dentin matrix protein 1. Meanwhile, DFCs as 1 source of dental stem cells possessed high potency in odontogenic differentiation in vitro. Moreover, similar dentinlike tissues were observed in all 3 groups in vivo. Conclusions: CNCCs, DFCs, and DPCs possessed different biological characteristics in odontogenic differentiation.