CLC transport proteins in plants
CLC transport proteins in plants
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DOI：
10.1016/j.febslet.2009.12.042
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发表时间：
2010-05
期刊：
影响因子：
3.5
通讯作者：
G. Zifarelli;Michael Pusch
中科院分区：
文献类型：
--
作者：
G. Zifarelli;Michael Pusch
硝酸盐在胞内细胞器中的区室化一直被认为是植物生理学的关键，但长期以来所涉及的蛋白质的分子身份仍然不清楚。在拟南芥中，AtClC-a最近已被证明是一个NO3-/H+逆向转运蛋白的硝酸盐运输到液泡的关键。AtClC-a是CLC蛋白家族的成员，其动物和细菌成员，包括通道和H+偶联的反向转运蛋白，先前仅涉及Cl−转运。尽管AtClC-a与其他CLC反向转运蛋白相比具有不同的NO3-对Cl−的选择性，但它具有相似的转运特性。其他CLC同源物已在拟南芥、烟草、水稻和大豆中克隆。
Nitrate compartmentalization in intracellular organelles has been long recognized as critical for plant physiology but the molecular identity of the proteins involved remained unclear for a long time. In Arabidopsis thaliana, AtClC-a has been recently shown to be a NO3-/H+antiporter critical for nitrate transport into the vacuoles. AtClC-a is a member of the CLC protein family, whose animal and bacterial members, comprising both channels and H+-coupled antiporters, have been previously implicated exclusively in Cl−transport. Despite the different NO3-over Cl−selectivity of AtClC-a compared to the other CLC antiporters, it has similar transport properties. Other CLC homologues have been cloned in Arabidopsis, tobacco, rice and soybean.