USE OF SERUM ANTIBODY AND LYSOZYME LEVELS FOR DIAGNOSIS OF LEPROSY AND TUBERCULOSIS
USE OF SERUM ANTIBODY AND LYSOZYME LEVELS FOR DIAGNOSIS OF LEPROSY AND TUBERCULOSIS
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DOI：
10.1128/jcm.30.5.1105-1110.1992
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发表时间：
1992-05-01
影响因子：
9.4
通讯作者：
LEFFORD, MJ
中科院分区：
文献类型：
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作者：
NEAR, KA;LEFFORD, MJ
活动性结核病和麻风病很难早期诊断，因为可检测的微生物很少，特异性免疫反应无法区分活动性和非活动性疾病。 我们开发了一种溶菌酶的免疫测定法，以观察血清溶菌酶水平是否可用于识别临床麻风或结核病患者。 溶菌酶的免疫测定法上级灵敏度和可靠性较低的标准酶测定法。 将溶菌酶测定法与结核分枝杆菌脂阿拉伯甘露聚糖（LAM）和M.麻风酚糖脂-1。 测试的血清来自埃塞俄比亚麻风病（少杆菌和多杆菌）和TB患者以及健康的埃塞俄比亚和美国对照。 溶菌酶检测能够检测更多的个人与结核病（19例患者的敏感性，100%）或麻风（36例患者的敏感性，86%）比任何抗体检测。 特别是，溶菌酶水平提高了一个较高的比例的少杆菌型麻风病患者（83%的17），对他们的抗体检测灵敏度较低; LAM IgG和酚糖脂-1 IgM水平分别提高了62和44%的16例患者。 这些数据表明，溶菌酶的测量可能是有用的分枝杆菌感染和其他慢性感染性肉芽肿病的诊断。
Active tuberculosis (TB) and leprosy are difficult to diagnose early because there are few organisms to detect and the specific immune response does not distinguish between active and inactive disease. We developed an immunoassay for lysozyme to see whether serum lysozyme levels could be used to identify individuals with clinical leprosy or TB. The immunoassay for lysozyme proved superior to standard enzyme assays that were less sensitive and reliable. The lysozyme assay was compared with assays for antibodies to Mycobacterium tuberculosis lipoarabinomannan (LAM) and M. leprae phenolic glycolipid-1. The sera tested were from Ethiopian leprosy (paucibacillary and multibacillary) and TB patients and from healthy Ethiopian and U.S. controls. The lysozyme assay was able to detect more of the individuals with TB (sensitivity, 100% for 19 patients) or leprosy (sensitivity, 86% for 36 patients) than either antibody assay. In particular, lysozyme levels were raised in a higher proportion of the paucibacillary leprosy patients (83% of 17), for whom the antibody assays were less sensitive; the LAM IgG and the phenolic glycolipid-1 IgM levels were raised in only 62 and 44% of 16 patients, respectively. The data suggest that lysozyme measurements may be useful in the diagnosis of mycobacterial infections and other chronic infectious granulomatoses.