In vitro testing of dental materials by means of macrophage cultures: II. Effects of particulate dental amalgams and their constituent phases on cultured macrophages.
In vitro testing of dental materials by means of macrophage cultures: II. Effects of particulate dental amalgams and their constituent phases on cultured macrophages.
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通过巨噬细胞培养对牙科材料进行体外测试：II。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
K. Nilner
中科院分区：
文献类型：
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作者：
S. Syrjänen;A. Hensten;K. Nilner
已知巨噬细胞在从组织中去除外来颗粒物质中起重要作用。当牙科汞合金粉末进入软组织时，由于巨噬细胞及其多核体对汞合金的细胞内降解，形成汞合金纹身。因此，在体外研究微粒汞合金及其各相对巨噬细胞的影响是可行的。比较的参数是吞噬率、细胞形态学变化和乳酸脱氢酶（LDH）释放，以证明质膜通透性。结果表明，除Sn 8Hg颗粒（γ 2相）和含γ 2的Revalloy外，所有合金均被巨噬细胞有效吞噬，并且在第一天细胞形态的改变轻微。在用颗粒状Ag2Hg3（γ 1相）和Revalloy处理1周的培养物中观察到显著的细胞损伤。合金处理后1小时，培养基中LDH活性略有增加。由于汞合金处理的LDH活性增加的顺序Dispersalloy小于Revalloy小于Sybraloy。腹腔内吞噬作用没有引起巨噬细胞的任何形态学变化，但吞噬作用的百分比减少。
It is known that macrophages play an important role in the removal of foreign particulate matter from tissue. When powdered dental amalgam is introduced into the soft tissues an amalgam tattoo is formed due to the intracellular degradation of amalgam by macrophages and their polykaryons. It was therefore feasible to study the effects of particulate amalgams as well as their individual phases on macrophages in vitro. The parameters compared were rate of the phagocytosis, changes of cellular morphology, and release of lactate dehydrogenase (LDH) to demonstrate plasma membrane permeability. It was shown that all the alloys except the Sn8Hg particles (gamma 2-phase) and gamma 2-containing Revalloy were effectively phagocytized by macrophages, and the alterations in cellular morphology were slight during the first day. Prominent cellular damage was seen in cultures treated with particulate Ag2Hg3 (gamma 1-phase) and Revalloy for 1 week. A slight increase in LDH activity in the medium was seen one hour after the alloy treatment. The LDH activities due to the amalgam treatment increased in the order Dispersalloy less than Revalloy less than Sybraloy. Intraperitoneal phagocytosis did not cause any morphological changes in macrophages, but the per cent of phagocytosis was diminished.