Patients with constipation-predominant irritable bowel syndrome (IBS) may have elevated serotonin concentrations in colonic mucosa as compared with diarrhea-predominant patients and subjects with normal bowel habits
Patients with constipation-predominant irritable bowel syndrome (IBS) may have elevated serotonin concentrations in colonic mucosa as compared with diarrhea-predominant patients and subjects with normal bowel habits
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DOI：
10.1159/000051888
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发表时间：
2001-01-01
期刊：
影响因子：
3.2
通讯作者：
Umeda, N
中科院分区：
文献类型：
--
作者：
Miwa, J;Echizen, H;Umeda, N
背景：5-羟色胺（5-HT）可能在人类结肠运动的调节中起重要作用。然而，结肠5-HT系统的改变是否参与肠易激综合征IBS的病理生理尚不清楚。研究方法：通过内镜活检从腹泻或便秘为主的IBS患者（分别为n = 7和n = 8）和正常排便习惯的受试者（n = 7）获得从升结肠到直肠的结肠粘膜标本，以确定IBS不同临床表现的患者是否具有不同的5-HT粘膜分布。采用反相高效液相色谱荧光检测法测定5-HT及其主要代谢产物5-羟基吲哚乙酸的组织浓度。结果如下：在所有研究组中，从直肠获得的平均粘膜5-HT浓度显著（p < 0.05）高于从结肠的更多头部区域获得的平均粘膜5-HT浓度。此外，从便秘型IBS患者中获得的总体平均粘膜5-HT浓度显著（p < 0.05）高于从对照受试者和腹泻型IBS患者中获得的浓度。三组间5-羟基吲哚乙酸浓度无显著差异。结论：在所有研究组中，结肠粘膜5-HT浓度均呈头尾向梯度上升。虽然便秘型IBS患者的结肠粘膜5-HT浓度高于腹泻型IBS患者和对照组，但结肠粘膜5-HT浓度升高是否是结肠动力异常的原因或结果仍需进一步研究。版权所有（C）2001 S. Karger AG，巴塞尔。
Background: Serotonin (5-HT) may play an important role in the regulation of colonic motility in humans. However, it is not known whether alterations in the colonic 5-HT system are involved in the pathophysiology of irritable bowel syndrome IBS. Methods: Colonic mucosal specimens ranging from the ascending colon to the rectum were obtained from patients with diarrhea- or constipation-predominant IBS (n = 7 and n = 8, respectively) and from subjects with normal bower habits (n = 7) by endoscopic biopsy in order to determine whether patients with different clinical manifestations of IBS have different mucosal disposition of 5-HT. The tissue concentrations of 5-HT and its major metabolite, 5-hydroxyin-doleacetic acid, were determined by reversed-phase high-performance liquid chromatography with fluorescence detection. Results: in all study groups, the mean mucosal 5-HT concentrations obtained from the rectum were significantly (p < 0.05) higher than those obtained from more cephalic regions of the colon. In addition, the overall mean mucosal 5-HT concentrations obtained from patients with constipation-predominant IBS were significantly (p < 0.05) higher than those obtained from the control subjects and patients with diarrhea-predominant IBS. No significant differences were observed in 5-hydroxyindoleacetic acid concentrations among the three groups. Conclusions: The mucosal 5-HT concentrations in the colon showed an ascending cephalocaudal gradient in all study groups. Although the mucosal 5-HT concentrations were elevated in patients with constipation-predominant IBS as com pa red with those with diarrhea-predominant IBS and the control subjects, further stud ies a re necessary to determine whether the elevated mucosal 5-HT is a cause or a result of abnormal colonic motility. Copyright (C) 2001 S. Karger AG, Basel.