Clots retrieved by mechanical thrombectomy from acute ischemic stroke patients show no evidence of bacteria
Clots retrieved by mechanical thrombectomy from acute ischemic stroke patients show no evidence of bacteria
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DOI：
10.1177/1591019918819707
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发表时间：
2019-10-01
影响因子：
1.7
通讯作者：
Kallmes, David F.
中科院分区：
文献类型：
--
作者：
Khashim, Zenith;Fitzgerald, Sean;Kallmes, David F.
背景细菌和细菌成分与凝血因子的激活和血凝块的形成有关。本研究的目的是调查是否细菌种群存在于从患有大血管闭塞急性缺血性卒中（AIS）的患者中取出的凝块中。材料与方法收集了20例AIS患者的血凝块样本，这些患者接受了机械血栓切除术取血凝块。记录患者临床人口统计学详情。通过标准和实时聚合酶链反应（PCR）分析细菌16S rDNA的表达。进行革兰氏染色以鉴定革兰氏阳性菌和革兰氏阴性菌。结果20例AIS患者血凝块中均未检测到16SrDNA的表达。革兰氏染色结果显示凝块样品中不存在革兰氏阳性或革兰氏阴性细菌的表达。结论本研究未发现AIS患者血凝块中存在细菌。
Background Bacteria and bacterial components have been associated with the activation of coagulation factors and initiating the blood clot formation. The aim of this study was to investigate whether bacterial populations are present in clots retrieved from patients that have suffered a large vessel occlusion acute ischemic stroke (AIS). Materials and methods Clot samples were collected from 20 AIS patients who underwent clot retrieval with mechanical thrombectomy. Patient clinical demographic details were noted. Expression of bacterial 16S rDNA was analyzed by standard and real-time polymerase chain reaction (PCR). Gram staining was performed to identify Gram-positive and Gram-negative bacteria. Results Both the real-time and standard PCR demonstrated no expression of 16S rDNA in any of the 20 clots samples from AIS patients. Gram staining results showed no expression of Gram-positive or Gram-negative bacteria present in the clot samples. Conclusion Our current study found no bacteria populations in the clots of AIS patients.