Quantitation of viral load using real-time amplification techniques
Quantitation of viral load using real-time amplification techniques
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DOI：
10.1006/meth.2001.1264
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发表时间：
2001-12-01
期刊：
影响因子：
4.8
通讯作者：
Niesters, HGM
中科院分区：
文献类型：
--
作者：
Niesters, HGM
实时PCR扩增技术目前用于确定临床样本中越来越多靶点的病毒载量。实时PCR减少了扩增后产生结果所需的时间。内部开发的PCR和基于核酸序列的扩增（NASBA）的系统结合几种检测策略正在临床诊断环境中使用。这些检测在疾病管理中的重要性仍处于探索阶段。尽管这些技术具有精确测量临床样品中的DNA和RNA的隐含能力，但必须解决与标准化和质量控制相关的问题，以使这些技术能够在分子诊断中常规实施。(C)2001 Elsevier Science（美国）。
Real-time PCR amplification techniques are currently used to determine the viral load in clinical samples for an increasing number of targets. Real-time PCR reduces the time necessary to generate results after amplification. In-house developed PCR and nucleic acid sequence-based amplification (NASBA)-based systems combined with several detection strategies are being employed in a clinical diagnostic setting. The importance of these assays in disease management Is still In an exploration phase. Although these technologies have the implicit capability of accurately measuring DNA and RNA In clinical samples, issues related to standardization and quality control must be resolved to enable routine implementation of these technologies in molecular diagnostics. (C) 2001 Elsevier Science (USA).