Comparison of oocyte mRNA localization patterns in sterlet Acipenser ruthenus and African clawed frog Xenopus laevis
Comparison of oocyte mRNA localization patterns in sterlet Acipenser ruthenus and African clawed frog Xenopus laevis
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黑氏鲟和非洲爪蛙非洲爪蟾卵母细胞 mRNA 定位模式的比较
DOI：
10.1002/jez.b.22802
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
V. Kašpar
中科院分区：
文献类型：
--
作者：
K. Pocherniaieva;Monika Šídová;M. Havelka;Taiju Saito;M. Pšenička;Radek Sindelka;V. Kašpar
在卵母细胞中，RNA定位具有关键意义，因为蛋白质在特定亚细胞结构域中的组装对胚胎发育至关重要。mRNA分子的分布可以识别和表征定位的转录物。本研究的目的是阐明原始生殖细胞在卵母细胞体平面中的起源，并揭示通过定位RNA的细胞谱系的产生。通过qPCR断层扫描研究了12种选定的mRNA在鲟鱼卵母细胞中的分布，并与非洲爪蟾中已知的mRNA定位模式进行了比较。我们研究了两个基因簇在鲟鱼卵母细胞的卵质中的分布，这两个基因簇沿着动物-植物轴，都显示出明确的细胞内梯度模式非常相似，它们在青蛙卵母细胞中的分布。我们阐明了定位鲟鱼卵种质标记属于植物组的mRNA。与此相反，编码otx 1，wnt 11和veg 1的mRNA被发现定位于鲟鱼动物半球，分布在两栖动物的植物半球。Actinopaltii和Sarcopaltii，硬骨鱼类脊椎动物的两个主要谱系，分裂约476 Ma（布莱尔和赫奇斯，），尽管基础谱系共享保守的生物学特征。鲟形目是Actinopteri的最基本的生活谱系之一，已经进化了大约200 Ma（Bemis，比尔斯坦，& Waldman，），与现代两栖动物同期（Roelants等人，).
In oocytes, RNA localization has critical implications, as assembly of proteins in particular subcellular domains is crucial to embryo development. The distribution of mRNA molecules can identify and characterize localized transcripts. The goal of this study was to clarify the origin of primordial germ cells in the oocyte body plan and to reveal the generation of cell lineages by localized RNAs. The distribution of 12 selected mRNAs in sterlet Acipenser ruthenus oocytes was investigated by qPCR tomography and compared with known patterns of mRNA localization in Xenopus laevis. We investigated the distribution of two gene clusters in the ooplasm along the animal-vegetal axis of the sturgeon oocyte, both of which showed clearly defined intracellular gradient pattern remarkably similar to their distribution in the frog oocyte. We elucidated the localization of sturgeon egg germplasm markers belonging to the vegetal group of mRNAs. The mRNAs coding otx1, wnt11, and veg1 found to be localized in the sturgeon animal hemisphere are, in contrast, distributed in the vegetal hemisphere in amphibian. Actinopterygii and Sarcopterygii, two major lineages of osteichthyan vertebrates, split about 476 Ma (Blair & Hedges, ), albeit basal lineages share conserved biological features. Acipenseriformes is one the most basal living lineages of Actinopterygii, having evolved about 200 Ma (Bemis, Birstein, & Waldman, ), contemporaneous with modern amphibians (Roelants et al., ).
生发颗粒和局部 RNA 通过信使运输组织者途径递送至非洲爪蟾卵母细胞的植物皮层，这是通过线粒体云的定向扩张而发生的。
DOI：
10.1387/ijdb.041906kw
发表时间：
2005
期刊：
The International journal of developmental biology
影响因子：
--
作者：
Wilk,Katarzyna;Bilinski,Szczepan;Dougherty,MatthewT;Kloc,Malgorzata
通讯作者：
Kloc,Malgorzata