Early stage mechanical remodeling of collagen surrounding head and neck squamous cell carcinoma spheroids correlates strongly with their invasion capability
Early stage mechanical remodeling of collagen surrounding head and neck squamous cell carcinoma spheroids correlates strongly with their invasion capability
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DOI：
10.1016/j.actbio.2018.11.046
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发表时间：
2019-01-15
影响因子：
9.7
通讯作者：
Chiou, Arthur
中科院分区：
文献类型：
--
作者：
Chen, Yin-Quan;Kuo, Jean-Cheng;Chiou, Arthur
间质胶原的机械重塑，如由侵入的癌细胞产生的胶原基质的重新定向和变形，在癌症侵袭和转移的进展中起重要作用。在这项研究中，我们应用延时显微镜结合粒子位移映射来分析头颈部鳞状细胞癌细胞（HNSCC），OECM-1和SAS的单个球体周围的胶原蛋白的时间依赖性收缩和膨胀变形，因为癌细胞从球体脱离并侵入周围的3D胶原蛋白基质。我们的研究结果表明，高度侵袭性HNSCC球体，刺激表皮生长因子（EGF），产生了强烈的收缩变形周围的胶原蛋白在非常早期的阶段，并对齐的胶原纤维径向相对于球体的中心。这种由HNSCC球体产生的初始胶原收缩变形与随后的癌细胞侵袭的总体程度具有强的正相关性;因此，它可以作为HNSCC球体侵袭能力的早期指标。我们观察到头颈部鳞状细胞癌细胞（HNSCC）球体周围胶原蛋白的初始收缩变形程度在早期促进癌细胞侵入周围ECM中起着不可或缺的作用。我们的研究结果表明，更具侵袭性的HNSCC球体产生更大程度的初始胶原收缩，以对齐周围的胶原并促进癌细胞侵袭。由HNSCC球状体产生的这种初始胶原收缩变形与癌细胞侵袭的总体程度具有强正相关性;因此，它可以作为HNSCC球状体侵袭能力的早期指标。(C)2018 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Mechanical remodeling of stromal collagen, such as reorientation and deformation of collagen matrix, generated by invading cancer cells, plays an important role in the progression of cancer invasion and metastasis. In this study, we applied time-lapse microscopy in conjunction with particle displacement mapping to analyze time-dependent contraction and expansion deformations of collagen surrounding individual spheroids of head and neck squamous cell carcinoma cells (HNSCC), OECM-1 & SAS, as the cancer cells detached from the spheroid and invaded into the surrounding 3D collagen matrix. Our results revealed that highly-invasive HNSCC spheroids, stimulated by epidermal growth factor (EGF), generated a strong contraction deformation of the surrounding collagen in the very early stage, and aligned the collagen fibers radially with respect to the center of the spheroid. This initial collagen contraction deformation generated by the HNSCC spheroid bears a strong positive correlation with the overall extent of subsequent cancer cells invasion; hence, it may serve as an early indicator of the invasion capability of the HNSCC spheroids.Statement of SignificanceMechanical remodeling of extracellular matrix (ECM) generated by cancer cells plays an important role in the progression of cancer invasion and metastasis. We observed that the extent of initial contraction deformation of collagen surrounding a head and neck squamous cell carcinoma cell (HNSCC) spheroid played an indispensable role in early stage to promote cancer cells invasion into the surrounding ECM. Our results revealed that more invasive HNSCC spheroids generated a larger extent of initial collagen contraction to align the surrounding collagen and to promote cancer cells invasion. This initial collagen contraction deformation generated by the HNSCC spheroids bears a strong positive correlation with the overall extent of cancer cells invasion; hence, it may serve as an early indicator of the invasion capability of the HNSCC spheroids. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.