BINDING OF A SPECIFIC LIGAND INHIBITS IMPORT OF A PURIFIED PRECURSOR PROTEIN INTO MITOCHONDRIA
BINDING OF A SPECIFIC LIGAND INHIBITS IMPORT OF A PURIFIED PRECURSOR PROTEIN INTO MITOCHONDRIA
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DOI：
10.1038/322228a0
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发表时间：
1986-07-17
期刊：
影响因子：
64.8
通讯作者：
SCHATZ, G
中科院分区：
文献类型：
--
作者：
EILERS, M;SCHATZ, G
叶酸拮抗剂甲氨蝶呤阻断与线粒体前序列融合的小鼠二氢叶酸还原酶输入线粒体。甲氨蝶呤不掩盖前序列，但稳定二氢叶酸还原酶部分。它并不禁止进口前序列所来源的真实前体。这表明二氢叶酸还原酶必须至少部分解折叠才能穿过线粒体膜。
Methotrexate, a folate antagonist, blocks import into mitochondria of mouse dihydrofolate reductase fused to a mitochondrial presequence. Methotrexate does not mask the presequence, but stabilizes the dihydrofolate reductase moiety. It does not inhibit import of the authentic precursor from which the presequence is derived. This suggests that dihydrofolate reductase must at least partly unfold in order to be transported across mitochondrial membranes.