Thrombin and Chymotrypsin Interactions with Thrombospondin a
Thrombin and Chymotrypsin Interactions with Thrombospondin a
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凝血酶和胰凝乳蛋白酶与血小板反应蛋白 a 的相互作用
DOI：
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发表时间：
1986
影响因子：
5.2
通讯作者：
Laura H. Derick
中科院分区：
文献类型：
--
作者：
J. Lawler;JOSEPH E. Connolly;P. Ferro;Laura H. Derick
血栓形成蛋白是一种高分子量糖蛋白，具有与肝素结合的能力。' fibr~nectin'.~纤维蛋白原、纤溶酶原、富含组氨酸糖蛋白、V型胶原、层粘连蛋白、钙调蛋白和硫酸化糖脂。“虽然这些相互作用的功能意义尚不清楚，但其中一些可能导致凝血酶敏感蛋白掺入细胞外。凝血酶敏感蛋白已被证明存在于由内皮细胞、平滑肌细胞和成纤维细胞产生的细胞外基质中。McKeown-Longo等人13已经表明，在培养的人胚肺成纤维细胞中加入的一些放射性标记的血小板反应蛋白被掺入细胞外基质中。与纤连蛋白相反，基质结合的血小板反应蛋白在数小时内缓慢降解。最近的研究表明，培养物中细胞的血小板反应蛋白生物合成与细胞密度成正比，并受血小板衍生生长因子和糖胺聚糖的调节。“*16此外，在新生儿皮肤的人内皮细胞培养物中可以检测到血小板反应蛋白掺入细胞外基质，但在成人皮肤中不能检测到。“这些数据表明，血小板反应蛋白可能在细胞外基质的形成中具有特定的作用，并可能受到生物合成表型变化的影响。发展中的纤维蛋白凝块可能代表细胞外基质的特殊例子。通过测定与这些凝块相关的放射性标记的血小板反应蛋白的量和免疫荧光，可以证明血小板反应蛋白掺入纤维蛋白和血浆凝块中。血栓反应蛋白在凝块中的分布随时间变化的研究表明，大多数血栓反应蛋白仅与凝块短暂相关。“Bale等报道了当纤维蛋白形成时，凝血酶敏感蛋白被结合到凝块中。Tuszynski等人报道，如果因子XIII的交联被抑制，更多的血小板反应蛋白被掺入纤维蛋白凝块中。凝血酶敏感蛋白在因子XIII存在下共价结合到高分子量复合物中，表明
Thrombospodin is a high molecular weight glycoprotein with the ability to bind to heparin,'.' fibr~nectin,'.~ fibrin~gen,"~ plasminogen,6 histidine-rich glycoprotein,' type V collagen,' laminin,' c a l c i~m,~ and sulfated glycolipids." Although the functional significance of these interactions is not known, some of them probably result in the incorporation of thrombospondin into extracellular Thrombospondin has been shown to be present in the extracellular matrices produced by endothelial cells, smooth muscle cells, and fibroblasts in c~ l tu re .~ ' ' ~ McKeown-Longo et aL13 have shown that some of the radiolabeled thrombospondin that is added to human embryonic lung fibroblasts in culture is incorporated into the extracellular matrix. In contrast to fibronectin, the matrix-bound thrombospondin is slowly degraded over a period of hours.I3 Recent studies indicate that thrombospondin biosynthesis by cells in culture is proportional to cell density and is modulated by platelet-derived growth factor and glycosaminoglycans."*16 In addition, the incorporation of thrombospondin into the extracellular matrix can be detected in cultures of human endothelial cells from neonatal skin, but not from adult skin." These data suggest that thrombospondin may have a specific role in the formation of the extracellular matrix and may be affected by variations in biosynthetic phenotype. The developing fibrin clot may represent a specialized example of an extracellular matrix. The incorporation of thrombospondin into fibrin and plasma clots can be demonstrated by determining the amount of radiolabeled thrombospondin that is associated with these clots and by immunofl~orescence.~~~~' Studies of thrombospondin distribution in the clot as a function of time suggest that the majority of thrombospondin is only transiently associated with the clot." Bale et al. I' have reported that thrombospondin is incorporated into the clot while the fibrin is forming. Tuszynski et have reported that more thrombospondin is incorporated into fibrin clots if cross-linking by factor XIII, is inhibited. Thrombospondin is covalently incorporated into high molecular weight complexes in the presence of factor XIII., suggesting that
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血小板血小板反应蛋白与纤溶酶原的复合形成。
DOI：
10.1172/jci111578
发表时间：
1984
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Silverstein,RL;Leung,LL;Harpel,PC;Nachman,RL
通讯作者：
Nachman,RL
DOI：
10.1073/pnas.80.4.998
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
JAFFE, EA;RUGGIERO, JT;MOSHER, DF
通讯作者：
MOSHER, DF
DOI：
10.1016/s0021-9258(18)33117-x
发表时间：
1983-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
S. Almeda;R. Rosenberg;D. Bing
通讯作者：
S. Almeda;R. Rosenberg;D. Bing
血小板反应蛋白在原位形成的凝块中定位。
影响因子：
20.3
作者：
Murphy-Ullrich,JE;Mosher,DF
通讯作者：
Mosher,DF
人血小板血小板反应蛋白与纤维蛋白原的相互作用。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Tuszynski,GP;Srivastava,S;Switalska,HI;Holt,JC;Cierniewski,CS;Niewiarowski,S
通讯作者：
Niewiarowski,S