Diagnostic value of fluorescein-labeled chitinase staining in formalin-fixed and paraffin-embedded tissues of fungal disease
Diagnostic value of fluorescein-labeled chitinase staining in formalin-fixed and paraffin-embedded tissues of fungal disease
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荧光素标记的几丁质酶染色对福尔马林固定和石蜡包埋的真菌病组织的诊断价值
DOI：
10.1093/mmy/myz035
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发表时间：
2020-01-01
期刊：
影响因子：
2.9
通讯作者：
Yu, Jin
中科院分区：
文献类型：
--
作者：
Shao, Jin;Song, Yinggai;Yu, Jin
常见的组织病理学技术用于诊断真菌感染，但组织标本中真菌病的诊断鉴定通常很困难，特别是当真菌很少出现在标本中时。本研究的目的是评价荧光素标记几丁质酶染色法在福尔马林固定和石蜡包埋（FFPE）组织中的应用。我们研究了79例确诊为真菌病的患者的存档FFPE组织，包括38例孢子虫病和41例其他真菌感染。组织切片进行过碘酸-希夫（PAS）染色，Gomori的乌洛托品银（GMS）染色，和荧光素标记的几丁质酶染色，以检测真菌元素。培养和/或hemoxylineosin阳性样本被用来估计每种染色方法的诊断灵敏度，结果显示PAS，GMS和荧光素标记的几丁质酶染色的灵敏度分别为50.6%，70.9%和68.4%。除孢子虫病和着色芽生菌病外，所有真菌感染的三种染色结果相同。黄绿霉素标记几丁质酶染色法对孢子虫病敏感性高，而对着色芽生菌病鼠形细胞的检测效果较差。荧光素标记几丁质酶染色的敏感性高于PAS染色，与GMS染色的敏感性相近。因此，我们的研究结果表明，荧光素标记几丁质酶染色是一个潜在的有用的诊断工具，在真菌感染的诊断。
Common histopathologic techniques are used to diagnose fungal infections, but the diagnostic identification of mycoses in tissue specimens is often difficult, particularly when fungi rarely occur in a specimen. The aim of this study was to evaluate the application of fluorescein-labeled chitinase staining to formalin-fixed and paraffin-embedded (FFPE) tissues. We studied 79 archival FFPE tissues from patients diagnosed with fungal disease, including 38 cases of sporotrichosis and 41 cases of other fungal infections. The tissue sections were subjected to periodic acid-Schiff (PAS) staining, Gomori's methenamine silver (GMS) staining, and fluorescein-labeled chitinase staining to detect fungal elements. Culture- and/or hematoxylineosin-positive samples were used to estimate the diagnostic sensitivity of each staining method, with the results showing that PAS, GMS, and fluorescein-labeled chitinase staining had sensitivities of 50.6, 70.9, and 68.4%, respectively. The three staining results were the same for all fungal infections except for sporotrichosis and chromoblastomycosis. Fluorescein-labeled chitinase staining exhibited high sensitivity in cases of sporotrichosis and poor performance in detecting muriform cells of chromoblastomycosis. On the whole, the sensitivity of fluorescein-labeled chitinase staining was greater than that of PAS and similar to that of GMS staining. Therefore, the results of our study suggest that fluorescein-labeled chitinase staining is a potentially useful diagnostic tool in the diagnosis of fungal infections.