Methods to culture, differentiate, and characterize neural stem cells from the adult and embryonic mouse central nervous system.
Methods to culture, differentiate, and characterize neural stem cells from the adult and embryonic mouse central nervous system.
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DOI：
10.1007/978-1-62703-128-8_30
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Reynolds, Brent A
中科院分区：
文献类型：
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作者：
Louis, Sharon A;Mak, Carmen K H;Reynolds, Brent A
自从在胚胎和成年哺乳动物中枢神经系统（CNS）中发现神经干细胞（NSC）以来，越来越多的组织培养基和方案用于体外研究和表征NSCs及其后代的功能。1992年引入的其中一种培养系统被称为神经球测定法，它已被广泛用于分离、扩增、分化甚至量化NSC种群。几年后，由于它作为一种体外定量测定NSC频率的方法的应用受到限制，一种新的基于半固体的单步测定法——神经集落形成细胞（NCFC）测定法被开发出来，以准确测量NSC数量。nfc检测允许通过它们产生的菌落大小（即它们的增殖潜力）来区分NSCs和祖细胞。这些组织培养工具的发展和持续改进将促进NSCs在治疗应用方面的进一步发展。
Since the discovery of neural stem cells (NSC) in the embryonic and adult mammalian central nervous system (CNS), there have been a growing numbers of tissue culture media and protocols to study and functionally characterize NSCs and its progeny in vitro. One of these culture systems introduced in 1992 is referred to as the Neurosphere Assay, and it has been widely used to isolate, expand, differentiate and even quantify NSC populations. Several years later because its application as a quantitative in vitro assay for measuring NSC frequency was limited, a new single-step semisolid based assay, the Neural Colony Forming Cell (NCFC) assay was developed to accurately measure NSC numbers. The NCFC assay allows the discrimination between NSCs and progenitors by the size of colonies they produce (i.e., their proliferative potential). The evolution and continued improvements made to these tissue culture tools will facilitate further advances in the promising application of NSCs for therapeutic use.