COLONY-STIMULATING FACTOR-I AND C-FMS EXPRESSION IN HUMAN ENDOMETRIAL TISSUES AND PLACENTA DURING THE MENSTRUAL-CYCLE AND EARLY-PREGNANCY
COLONY-STIMULATING FACTOR-I AND C-FMS EXPRESSION IN HUMAN ENDOMETRIAL TISSUES AND PLACENTA DURING THE MENSTRUAL-CYCLE AND EARLY-PREGNANCY
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DOI：
10.1210/jcem-73-4-746
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发表时间：
1991-10-01
影响因子：
5.8
通讯作者：
TURNER, T
中科院分区：
文献类型：
--
作者：
KAUMA, SW;AUKERMAN, SL;TURNER, T
集落刺激因子-1(CSF-1)是一种由单核细胞、巨噬细胞、成纤维细胞和内皮细胞产生的生长因子，通过其受体c-FMS参与胎盘的功能调节和生长。在这项研究中，我们用放射免疫法检测了正常月经周期和早孕的人子宫内膜、蜕膜和胎盘组织中CSF-1的组织水平，并用Northern印迹分析了CSF-1和c-FMS mRNA的相对表达。所有的子宫内膜、蜕膜和胎盘组织都显示出可提取的免疫活性CSF-1，并表达4.0kb的CSF-1mRNA种类。早孕蜕膜组织中CSF-1mRNA的表达水平高于增生期(3.2倍；P<；0.01)和分泌期(2.4倍；P<；0.01)，而增殖期和分泌期子宫内膜组织中的表达水平相近。早孕蜕膜组织中可提取的CSF-1免疫活性水平是增生期子宫内膜组织的3.2倍(P<；0.05)，分泌期子宫内膜组织中是增生期子宫内膜组织的2.9倍(P<；0.05)，而早孕蜕膜组织和分泌期子宫内膜组织中的免疫活性CSF-1水平相似。C-FMS mRNA在所有子宫内膜和早孕蜕膜组织中均有表达，在月经周期和早孕中变化不大。胎盘组织中CSF-1和c-FMS基因表达水平与胎龄呈正相关。这些结果表明，在植入期间和早孕期间，在母胎界面发现的组织中局部产生了更多的CSF-1。这种增加的CSF-1的产生可能通过c-FMS在这些组织中的存在而在蜕膜功能和胎盘生长中发挥作用。
Colony-stimulating factor-1 (CSF-1), a growth factor produced by monocytes, macrophages, fibroblasts, and endothelial cells, has been implicated in the functional regulation and growth of the murine placenta through the presence of the CSF-1 receptor, c-fms, found in this tissue. In this study we examined the tissue levels of CSF-1 by RIA and the relative expression of CSF-1 and c-fms mRNA by Northern blot analysis in human endometrial, decidual, and placental tissues during the normal menstrual cycle and early pregnancy. All endometrial, decidual, and placental tissues demonstrated extractable immunoreactive CSF-1 and expressed the 4.0-kilobase CSF-1 mRNA species. First trimester decidual tissue expressed higher levels of CSF-1 mRNA than proliferative (3.2-fold higher; P < 0.01) or secretory (2.4-fold higher; P < 0.01) endometrial tissues, whereas proliferative and secretory endometrial tissues expressed similar levels of CSF-1 mRNA. Tissue extractable levels of immunoreactive CSF-1 were 3.2-fold (P < 0.05) higher in first trimester decidual tissue and 2.9-fold (P < 0.05) higher in secretory endometrial tissue compared to levels in proliferative endometrial tissue, whereas first trimester decidua and secretory endometrial tissues had similar levels of immunoreactive CSF-1. There was expression of c-fms mRNA in all endometrial and first trimester decidual tissue samples, with little change during the menstrual cycle and early pregnancy. In placenta, there was a positive correlation of increasing CSF-1 and c-fms mRNA expression with increasing gestational age. These results suggest that there is increased local production of CSF-1 in tissues found at the maternal-fetal interface during the time of implantation and early pregnancy. This increased production of CSF-1 may play a role in decidual function and placental growth through the presence of c-fms in these tissues.