Gene expression of 3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase in relation to androstenone, testosterone, and estrone sulphate in gonadally intact male and castrated pigs
Gene expression of 3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase in relation to androstenone, testosterone, and estrone sulphate in gonadally intact male and castrated pigs
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DOI：
10.2527/jas.2007-0087
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发表时间：
2007-10-01
影响因子：
3.3
通讯作者：
Lundstrom, K.
中科院分区：
文献类型：
--
作者：
Chen, G.;Bourneuf, E.;Lundstrom, K.
雄甾烯酮是引起猪腥味的主要物质之一，3 β-羟基类固醇脱氢酶（3 β HSD）可能参与其代谢。在这项研究中，3 β HSD和17 β-羟类固醇脱氢酶（17 β HSD）的基因表达通过真实的-时间PCR分析测定，并与雄甾烯酮、睾酮和硫酸雌酮（E1 S）的浓度相关。对基于高或低脂肪雄烯酮浓度（如通过HPLC预先确定）分类的性腺完整的雄性猪以及免疫去势和手术去势的雄性猪进行实验。脂肪中雄甾烯酮浓度高的雄性猪肝脏和睾丸中3 β HSD基因表达较低。此外，17 β HSD基因在肝脏中的表达，而不是在睾丸中，与脂肪雄甾烯酮浓度呈负相关。免疫去势和手术去势的雄性猪脂肪雄烯酮和血浆睾酮和E1 S的浓度检测不到，和去势程序诱导3 β HSD和17 β HSD基因表达的显着增加。3 β HSD基因的mRNA表达通常比17 β HSD基因的mRNA表达大得多。此外，脂肪雄烯酮与肝脏3 β HSD基因表达呈负相关肝组织17 β HSD基因表达与血浆E1 S呈负相关（Pearson相关系数r =-0.69; P < 0.05）（r =-0.95; P < 0.001），表明肝脏17 β HSD在性腺完整的雄性猪的雌激素代谢中起重要作用。性腺完整的雄性猪肝脏中3 β HSD和17 β HSD基因的表达量之间也存在较强的相关性（r = 0.86; P < 0.01），可能反映了这些基因相似的调控机制。
Androstenone is one of the main compound responsible for boar taint, and 3 beta- hydroxysteroid dehydrogenase ( 3 beta HSD) might be involved in its metabolism. In this study, the gene expression of 3 beta HSD and 17 beta- hydroxysteroid dehydrogenase ( 17 beta HSD) were determined by real- time PCR analysis and related to the concentrations of androstenone, testosterone, and estrone sulphate ( E1S). The experiments were performed on gonadally intact male pigs classified based on high or low fat androstenone concentrations, as predetermined by HPLC, as well as on immunocastrated and surgically castrated male pigs. The male pigs with high androstenone concentrations in fat had low 3 beta HSD gene expression in liver and testis. Moreover, the 17 beta HSD gene expression in liver, but not in testis, varied negatively with fat androstenone concentrations. Immunocastrated and surgically castrated male pigs had nondetectable concentrations of fat androstenone and plasma testosterone and E1S, and the castration procedure induced a significant increase of 3 beta HSD and 17 beta HSD gene expression. The mRNA expression was generally much greater from the 3 beta HSD than from the 17 beta HSD gene. Furthermore, fat androstenone was negatively correlated with liver 3 beta HSD gene expression ( Pearson correlation, r = - 0.69; P < 0.05), and the 17 beta HSD gene expression in liver was negatively correlated with plasma E1S ( r = - 0.95; P < 0.001), indicating an important role of liver 17 beta HSD in the estrogen metabolism of gonadally intact male pigs. Another strong correlation was found between 3 beta HSD and 17 beta HSD gene expression in liver of the gonadally intact male pigs ( r = 0.86; P < 0.01), possibly reflecting similar regulation mechanisms of these genes.