[Expression of RhoC and ROCK-1 and their effects on MAPK and Akt proteins in prostate carcinoma].
[Expression of RhoC and ROCK-1 and their effects on MAPK and Akt proteins in prostate carcinoma].
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DOI：
10.3760/cma.j.issn.0253-3766.2011.03.011
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发表时间：
2011-03
期刊：
影响因子：
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通讯作者：
Qiang Bu;Huaming Tang;Jian Tan;Xiao Hu;Dong-wen Wang
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文献类型：
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作者：
Qiang Bu;Huaming Tang;Jian Tan;Xiao Hu;Dong-wen Wang
目的检测RhoC和Rho激酶1（ROCK-1）在前列腺癌组织中的表达，探讨RhoC/ROCK-1在前列腺癌发病中的可能机制。方法对73例前列腺癌患者行前列腺穿刺活检或根治性切除术，取癌旁组织标本。RT-PCR检测RhoC/ROCK-1 mRNA的表达。Western blot和免疫组化检测RhoC/ROCK-1蛋白的表达。构建RhoC真核表达质粒，转染PC-3 M-2B 4细胞。Western blot检测p-MAPK和p-Akt。结果RhoC和ROCK-1 mRNA在前列腺癌组织中的表达率分别为72.6%（53/73）和68.5%（50/73），显著高于癌旁组织中的34.2%（25/73）和38.4%（28/73）（P < 0.01）。结果表明，RhoC/ROCK-1 mRNA表达与Gleason分级无明显相关性。而RhoC/ROCK-1 mRNA的表达与远处转移呈显著正相关。RhoC/ROCK-1蛋白在前列腺癌中的表达也高于相应癌旁组织，且与p-MAPK和p-Akt表达水平呈显著正相关。此外，转录本中p-MAPK和p-Akt的表达水平上调。结论RhoC和ROCK-1在前列腺癌组织中的表达水平高于癌旁组织，与远处转移呈显著正相关。RhoC/ROCK-1可能参与了前列腺癌的发生、侵袭和转移。
OBJECTIVE To detect the expression of RhoC and Rho kinase 1 (ROCK-1) in prostate carcinoma, and explore the possible mechanism of RhoC/ROCK-1 in the pathogenesis of prostate carcinoma. METHODS Tissue specimens from 73 patients with prostate carcinoma and corresponding paracancerous tissues were obtained by prostate cancer biopsy or radical prostatectomy. The expression of RhoC/ROCK-1 mRNA was detected by RT-PCR. Western blot and immunohistochemistry were performed to dertect the expression of RhoC/ROCK-1 protein. Eukaryotic expression plasmids of RhoC were constructed and transfected into PC-3M-2B4 cells. p-MAPK and p-Akt were detected by Western bolt. RESULTS The expression levels of RhoC and ROCK-1 mRNA in the prostate carcinomas were significantly higher than those in corresponding paracancerous tissues [72.6% (53/73) vs. 34.2% (25/73); 68.5% (50/73) vs. 38.4% (28/73), P < 0.01], respectively. The results indicated that RhoC/ROCK-1 mRNA expression had no significant correlation with Gleason grade. However, the expression of RhoC/ROCK-1 mRNA showed a significant positive correlation with distant metastasis. The RhoC/ROCK-1 protein expression in prostate cancer was also higher than corresponding paracancerous tissues, and showed a significant positive correlation with p-MAPK and p-Akt expression levels. In addition, p-MAPK and p-Akt expression levels were up-regulated in the transcripts. CONCLUSION Expression levels of RhoC and ROCK-1 in prostate carcinoma are higher than those in corresponding paracancerous tissues, showing a significant positive correlation with distant metastasis. RhoC/ROCK-1 may be involved in the development, invasion and metastasis of prostate carcinoma.