Structural insight into the interaction of ADP-ribose with the PARP WWE domains
Structural insight into the interaction of ADP-ribose with the PARP WWE domains
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DOI：
10.1016/j.febslet.2012.09.009
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发表时间：
2012-11-02
期刊：
影响因子：
3.5
通讯作者：
Muto, Yutaka
中科院分区：
文献类型：
--
作者：
He, Fahu;Tsuda, Kengo;Muto, Yutaka
WWE结构域通常在与泛素化或聚ADP核糖基化相关的蛋白质中被鉴定。关于WWE结构域的结构信息已获得的泛素化相关蛋白，如Deltex和RNF 146，但尚未为聚ADP核糖聚合酶（PARP）。在这里，我们确定了PARP 11和PARP 14的WWE域的解结构，并与RNF 146 WWE域的解结构进行了比较。NMR微扰实验揭示了它们的ADP-核糖识别模式的具体差异，这些差异与它们各自的生物活性相关。目前的结构信息揭示了ADP-核糖识别模式的PARP WWE结构域。(C)2012年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
The WWE domain is often identified in proteins associated with ubiquitination or poly-ADP-ribosylation. Structural information about WWE domains has been obtained for the ubiquitination-related proteins, such as Deltex and RNF146, but not yet for the poly-ADP-ribose polymerases (PARPs). Here we determined the solution structures of the WWE domains from PARP11 and PARP14, and compared them with that of the RNF146 WWE domain. NMR perturbation experiments revealed the specific differences in their ADP-ribose recognition modes that correlated with their individual biological activities. The present structural information sheds light on the ADP-ribose recognition modes by the PARP WWE domains. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.