TRIMERIC FORMS OF THE PHOTOSYSTEM-I REACTION-CENTER COMPLEX PRE-EXIST IN THE MEMBRANES OF THE CYANOBACTERIUM SPIRULINA-PLATENSIS
TRIMERIC FORMS OF THE PHOTOSYSTEM-I REACTION-CENTER COMPLEX PRE-EXIST IN THE MEMBRANES OF THE CYANOBACTERIUM SPIRULINA-PLATENSIS
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DOI：
10.1016/0014-5793(93)81685-s
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发表时间：
1993-11-08
期刊：
影响因子：
3.5
通讯作者：
KARAPETYAN, NV
中科院分区：
文献类型：
--
作者：
SHUBIN, VV;TSUPRUN, VL;KARAPETYAN, NV
从中温蓝藻螺旋藻[(1992)FEBS Lett]中分离得到了光系统I(PSI)的低聚和单体形式的叶绿素-蛋白质复合体。309、340-342]。电子显微镜分析表明，低聚物是形状和尺寸与嗜热蓝藻的三聚体相似的三聚体。分离的三聚体和单体的叶绿素比例为7：3。与单体相比，三聚体的PSI复合体含有在760 nm(77K)处发射的叶绿素，这一点也存在于螺旋藻的膜中，因此可以作为三聚体复合体的内源探针。三聚体的77K圆二色谱与螺旋藻膜的光谱比单体的光谱更相似。因此，螺旋藻细胞膜中存在并占据主导地位的是三聚体PSI复合体。
Oligomeric and monomeric forms of chlorophyll-protein complexes of photosystem I (PSI) have been isolated from the mesophilic cyanobacterium Spirulina [(1992) FEBS Lett. 309, 340-342]. Electron microscopic analysis of the complexes showed that the oligomeric form is a trimer of the shape and dimensions similar to those of the trimer from thermophilic cyanobacteria. The chlorophyl ratio in the isolated trimer and monomer was found to be 7:3. The trimeric form of PSI complex in contrast to the monomeric one contains the chlorophyll emitting at 760 nm (77K), which is also found in Spirulina membranes and therefore could be used as an intrinsic probe for the trimeric complex. The 77K circular dichroism spectrum of the trimeric form is much more similar to that of Spirulina membranes than the spectrum of the monomer. Thus, the trimeric PSI complexes exist and dominate in the Spirulina membranes.