Mechanical transduction by rat dorsal root ganglion neurons in vitro
Mechanical transduction by rat dorsal root ganglion neurons in vitro
复制标题
DOI：
10.1016/s0304-3940(99)00665-5
复制
发表时间：
1999-10-08
影响因子：
2.5
通讯作者：
Levine, JD
中科院分区：
文献类型：
--
作者：
McCarter, GC;Reichling, DB;Levine, JD
虽然一般认为体感神经纤维的机械敏感性是机械敏感离子通道激活的结果，但在背根节(DRG)神经元中从未观察到机械门控的全细胞电流。我们在培养的大鼠背根神经节神经元上进行了膜片钳实验，首次报道了体感神经元中的机械激活电流(I-MECH)。这种全细胞电流在大多数背根神经节神经元中观察到，而在非感觉交感神经节神经元中未观察到。I-MECH的电流-电压关系表明它是非选择性的阳离子电流。I-MECH对Gd阻断的敏感性表明，它可能是由在许多细胞类型中观察到的机械敏感的非选择性阳离子通道家族的成员介导的。对苯扎米的敏感性支持这一观点，并进一步表明该电流可能是由变性蛋白/上皮钠通道(DEG/ENaC)家族的一个成员介导的。(C)1999爱思唯尔爱尔兰科学有限公司。保留所有权利。
Although it is generally presumed that mechanical sensitivity of somatosensory nerve fibers results from the activation of mechanosensitive ion channels, a mechanically-gated whole-cell current has never been demonstrated in dorsal root ganglion (DRG) neurons, We performed patch clamp experiments on rat DRG neurons in culture, and report the first mechanically-activated current in somatosensory neurons (I-mech) This whole-cell current is observed in most dorsal root ganglion neurons but not in non-sensory sympathetic ganglion neurons. The current-voltage relation of I-mech indicates that it is a non-selective cation current. Sensitivity of I-mech to block by gadolinium suggests that it may be mediated by a member of a family of mechanosensitive non-selective cation channels observed in many cell types. Sensitivity to benzamil supports this idea, and further suggests that the current might be mediated by a member of the degenerin/epithelial sodium channel (DEG/ENaC) family. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.