Recent development and trends in sample extraction and preparation for mass spectrometric analysis of nucleotides, nucleosides, and proteins
Recent development and trends in sample extraction and preparation for mass spectrometric analysis of nucleotides, nucleosides, and proteins
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DOI：
10.1016/j.jpba.2018.08.030
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发表时间：
2018-11
影响因子：
3.4
通讯作者：
Tadashi Hayama;K. Ohyama
中科院分区：
文献类型：
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作者：
Tadashi Hayama;K. Ohyama
本文综述了用于核苷酸、核苷和蛋白质质谱分析的样品提取和制备技术的最新进展。已经开发了独特的材料和技术，用于通过使用各种亲和力的固相提取策略来高度选择性地提取核苷酸和核苷。然而，对于蛋白质，对病变组织的小规模切片（福尔马林固定、石蜡包埋的组织）的分析和对病变组织表面上的精确病变的直接分析（液体提取表面分析）已经成为该领域的重要进展。在本文中，我们重点介绍了这些技术和策略的最新发展。
This review describes the recent developments in sample extraction and preparation techniques for mass spectrometric analysis of nucleotides, nucleosides, and proteins. Unique materials and techniques have been developed for highly selective extraction of nucleotides and nucleosides by solid-phase extraction strategies using various affinities. However, for proteins, the analysis of small-scale sections of diseased tissues (formalin-fixed, paraffin-embedded tissues) and the direct analysis of an exact lesion on the surface of diseased tissues (liquid extraction surface analysis) have become important advances in this field. In this review, we focus on the latest developments of these techniques and strategies.