Function, expression, specificity, diversity and incompatibility of actinobacteriophage parABS systems.
Function, expression, specificity, diversity and incompatibility of actinobacteriophage parABS systems.
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DOI：
10.1111/mmi.13414
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发表时间：
2016-08
影响因子：
3.6
通讯作者：
Hatfull GF
中科院分区：
文献类型：
--
作者：
Dedrick RM;Mavrich TN;Ng WL;Cervantes Reyes JC;Olm MR;Rush RE;Jacobs-Sera D;Russell DA;Hatfull GF
放线菌门中超过180个单独的细菌感染宿主已经被测序，并由于它们相似的总体核苷酸序列和基因组结构而被分组为A簇。这些簇A是温带或温带亲本的衍生物，并且大多数在基因组中心附近具有含有整合酶基因和attP的整合盒。然而，约20%的p53缺乏整合盒，其被具有预测的分配功能的1.4 kbp片段取代，包括质粒样帕拉和parB基因。噬菌体RedRock在耻垢分枝杆菌中形成稳定的溶原菌，其中原噬菌体以2.4拷贝/染色体复制，并且分配系统赋予原噬菌体维持。parAB基因在RedRock感染M.但是一旦通过RedRock ParB与parS-L（parAB基因侧翼的两个着丝粒样位点之一）的结合建立溶原性，则被下调。RedRock parS-L和parS-R位点由ParB识别的8 bp基序的8个直接重复拷贝组成。放线菌噬菌体parABS盒跨越相当大的序列多样性和特异性，提供了一套用于分枝杆菌遗传学的工具。
More than 180 individual phages infecting hosts in the phylum Actinobacteria have been sequenced and grouped into Cluster A because of their similar overall nucleotide sequences and genome architectures. These Cluster A phages are either temperate or derivatives of temperate parents, and most have an integration cassette near the center of the genome containing an integrase gene and attP. However, about 20% of the phages lack an integration cassette, which is replaced by a 1.4 kbp segment with predicted partitioning functions, including plasmid-like parA and parB genes. Phage RedRock forms stable lysogens in Mycobacterium smegmatis in which the prophage replicates at 2.4 copies/chromosome and the partitioning system confers prophage maintenance. The parAB genes are expressed upon RedRock infection of M. smegmatis, but are down-regulated once lysogeny is established by binding of RedRock ParB to parS-L, one of two centromere-like sites flanking the parAB genes. The RedRock parS-L and parS-R sites are composed of eight directly repeated copies of an 8 bp motif that is recognized by ParB. The actinobacteriophage parABS cassettes span considerable sequence diversity and specificity, providing a suite of tools for use in mycobacterial genetics.