The brown seaweed Cystoseira schiffneri as a source of sodium alginate: Chemical and structural characterization, and antioxidant activities
The brown seaweed Cystoseira schiffneri as a source of sodium alginate: Chemical and structural characterization, and antioxidant activities
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DOI：
10.1016/j.fbio.2020.100873
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发表时间：
2021-01-09
期刊：
影响因子：
5.2
通讯作者：
Zouari, Nacim
中科院分区：
文献类型：
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作者：
Benslima, Abdelkarim;Sellimi, Sabrine;Zouari, Nacim
从不同季节（12月、4月、7月和9月）从突尼斯岛屿（Kerkennah，斯法克斯）采集的棕色海藻（Cystoseira puffneri）中提取海藻酸钠。对C.对希夫奈藻酸钠（CSSA）进行了表征。微量元素分析表明，氮和硫的藻酸盐馏分的情况下。所有藻酸盐分离物的糖醛酸（47.4-66.4%）和灰分（24.3-39.4%）高。ATR-FTIR、NMR和圆二色谱分析结果表明，CSSA均为聚古洛糖醛酸酯型。使用高效尺寸排阻色谱法测定的重均分子量显示范围为4.49至1230 kDa的变化。热重分析表明，CSSA在400 ℃之前是稳定的，除了9月份收集的CSSA，观察到其仅在200 ℃之前是稳定的。采用DPPH中心点自由基清除法、还原力测定法和Fe 2+螯合能力测定法，测定了不同季节CSSA的抗氧化活性。CSSA的抗氧化潜力随季节变化显着，控制抗氧化性能的主要因素是分子量。
Sodium alginates were extracted from brown seaweeds (Cystoseira schiffneri) collected from a Tunisian island (Kerkennah, Sfax) in different seasons (December, April, July and September). The structural features and antioxidant properties of C. schiffneri sodium alginates (CSSA) were characterized. Micro-elementary analysis showed the absence of nitrogen and sulfur in alginate fractions. All alginate isolates were high in uronic acids (47.4-66.4%) and ash (24.3-39.4%). ATR-FTIR, NMR and circular dichroism data showed that all CSSA were of the polyguluronate-type. Weight-average molecular weight determined using high performance size exclusion chromatography showed variations ranging from 4.49 to 1230 kDa. Thermogravimetric analysis suggested that CSSA were stable until 400 degrees C except for that collected in September, which was observed to only be stable until 200 degrees C. Antioxidant activity of CSSA from different seasons was measured using DPPH center dot radical-scavenging, reducing power and Fe2+ chelating ability assays. Antioxidant potential of CSSA varied significantly with respect to the season and the main factor controlling antioxidant properties was the molecular mass.