Crystal structure of the MazE/MazF complex: Molecular bases of antidote-toxin recognition
Crystal structure of the MazE/MazF complex: Molecular bases of antidote-toxin recognition
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DOI：
10.1016/s1097-2765(03)00097-2
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发表时间：
2003-04-01
期刊：
影响因子：
16
通讯作者：
Burley, SK
中科院分区：
文献类型：
--
作者：
Kamada, K;Hanaoka, F;Burley, SK
大肠杆菌染色体迷宫/MazF成瘾模块的结构已在1.7埃分辨率下确定。成瘾模块由稳定的毒素和不稳定的解毒剂蛋白组成，这些蛋白控制细菌细胞死亡。迷宫（解毒剂）和MazF（毒素）形成由交替的毒素和解毒剂同二聚体组成的线性异六聚体（MazF（2）-迷宫（2）-MazF（2））。迷宫同源二聚体含有一个β桶，两个延伸的C末端从该桶伸出，与类似于质粒编码的毒素CcdB和Kid的侧翼MazF同源二聚体相互作用。迷宫/MazF异六聚体结构证明解毒剂-毒素识别的机制对于染色体和质粒携带的成瘾模块是共同的，并且提供了对毒素功能、在毒素不存在下的解毒剂降解以及解毒剂/毒素复合物与启动子DNA结合的一般分子见解。
A structure of the Escherichia coli chromosomal MazE/MazF addiction module has been determined at 1.7 Angstrom resolution. Addiction modules consist of stable toxin and unstable antidote proteins that govern bacterial cell death. MazE (antidote) and MazF (toxin) form a linear heterohexamer composed of alternating toxin and antidote homodimers (MazF(2)-MazE(2)-MazF(2)). The MazE homodimer contains a beta barrel from which two extended C termini project, making interactions with flanking MazF homodimers that resemble the plasmid-encoded toxins CcdB and Kid. The MazE/MazF heterohexamer structure documents that the mechanism of antidote-toxin recognition is common to both chromosomal and plasmid-borne addiction modules, and provides general molecular insights into toxin function, antidote degradation in the absence of toxin, and promoter DNA binding by antidote/toxin complexes.