The ComRS-SigX pathway regulates natural transformation in Streptococcus ferus.
The ComRS-SigX pathway regulates natural transformation in Streptococcus ferus.
复制标题
ComRS-SigX 途径调节野性链球菌的自然转化。
DOI：
10.1101/2023.03.06.531454
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Federle,MichaelJ
中科院分区：
文献类型：
--
作者：
Rued,BrittaE;Federle,MichaelJ
通过自然转化吸收和掺入外源 DNA 的能力是某些链球菌物种的众所周知的特征，并且是一种能够快速获得抗菌耐药性的机制。在这里，我们描述了正在研究的物种野性链球菌也能够自然转化，并使用与变形链球菌中鉴定的系统类似的系统。变形链球菌自然转化受替代 sigma 因子 igX（也称为 comX）的控制，其表达由两种类型的肽信号诱导：CSP（能力刺激肽，由 comC 编码）和 XIP（sigX 诱导肽，由 comS 编码）。这些系统分别通过二元信号转导系统 ComDE 或 RRNPP 转录调节因子 ComR 诱导能力。蛋白质和核苷酸同源性搜索确定了 comRS 和 sigXinS 的推定直系同源物。 ferus，但不是 S. mutansblpRH（也称为 comDE）的同源物。我们证明了 S 中的自然转变。 ferusis 由含有 sigX 诱导肽 (XIP) 的小型双色氨酸诱导，类似于变形链球菌，并且需要 comRandsigXorthologs 的存在才能有效转化。此外，我们发现自然转化是在 S 中诱导的。 ferus 与 S. mutans 的天然 XIP 和 XIP 变体均相同，这意味着这两个物种之间的串扰是可能的。该过程已被用来构建 S 中的基因缺失。 ferusand 提供了一种对这一待研究物种进行基因操纵的方法。重要性自然转化是细菌吸收 DNA 并获得新遗传性状的过程，包括与抗生素耐药性相关的遗传性状。这项研究表明，正在研究的野性链球菌能够利用肽-信息素系统进行自然转化，就像之前在变形链球菌中发现的那样，并为未来有关该生物体的研究提供了框架。
The ability to take up and incorporate foreign DNA via natural transformation is a well-known characteristic of some species of Streptococcus, and is a mechanism that rapidly allows for the acquisition of antibacterial resistance. Here, we describe that the understudied species Streptococcus ferus is also capable of natural transformation and uses a system analogous to that identified in Streptococcus mutans. S. mutans natural transformation is under the control of the alternative sigma factorsigX(also known ascomX), whose expression is induced by two types of peptide signals: CSP (competencestimulatingpeptide, encoded bycomC) and XIP (sigX-inducingpeptide, encoded bycomS). These systems induce competence via either the two-component signal-transduction system ComDE or the RRNPP transcriptional regulator ComR, respectively. Protein and nucleotide homology searches identified putative orthologs ofcomRSandsigXinS. ferus, but not homologs of S. mutansblpRH(also known ascomDE). We demonstrate that natural transformation inS. ferusis induced by a small, double-tryptophan containing sigX-inducing peptide (XIP), akin to that of S. mutans, and requires the presence of thecomRandsigXorthologs for efficient transformation. Additionally, we find that natural transformation is induced inS. ferusby both the native XIP and the XIP variant of S. mutans, implying that cross talk between the two species is possible. This process has been harnessed to construct gene deletions inS. ferusand provides a method to genetically manipulate this understudied species.IMPORTANCENatural transformation is the process by which bacteria take up DNA and allows for acquisition of new genetic traits, including those involved in antibiotic resistance. This study demonstrates that the understudied species Streptococcus ferus is capable of natural transformation using a peptide-pheromone system like that previously identified in Streptococcus mutans and provides a framework for future studies concerning this organism.