The analysis of the human plasma N-glycome in endometriosis patients
The analysis of the human plasma N-glycome in endometriosis patients
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DOI：
10.1016/j.ejogrb.2013.08.008
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发表时间：
2013-11-01
影响因子：
2.6
通讯作者：
Oehmke, F.
中科院分区：
文献类型：
--
作者：
Berkes, E.;Muzinic, A.;Oehmke, F.
目的：研究设计：在一项病例对照研究中，从2008年至2011年在I.布达佩斯的Semmelweis大学接受诊断性或手术性腹腔镜检查的患者中收集血液样本。妇产科。从这些患者中，选择92例子宫内膜异位症患者（30例I-II期和62例III-IV期，共18例深部浸润病例）和62例对照进行聚糖分析。放行后，对血浆N-聚糖进行亲水相互作用高效液相色谱分析，得到19个色谱聚糖峰（GP）。比较研究组之间GP的丰度。统计分析的非参数检验，Mann-Whitney-U检验，used.Results：我们发现，在子宫内膜异位症患者的GP 1和GP 14，GP 17和GP 18的增加有统计学意义的减少。后一个峰由在炎症过程和恶性肿瘤中起作用的聚糖组成。我们还发现对照组和不同子宫内膜异位症分期组之间的GP 2、GP 4、GP 6和GP 9存在显著差异。GP 2、GP 4和GP 6中观察到的改变可能与IgG分子聚糖分支的糖基化和重塑改变有关。GP 9的改变可能与转铁蛋白糖基化的改变有关。此外，我们检测到一个非常显着的减少GP 1在患者与深部浸润性子宫内膜异位症与controls.Conclusions相比：这是第一次分析血浆N-糖在子宫内膜异位症。观察到的GP 14、GP 17和GP 18以及GP 2、GP 4、GP 6和GP 9的变化为疾病的病理生理学提供了新的方面，并且GP 1的改变可能在未来作为新的潜在标记物。(C)2013爱思唯尔爱尔兰有限公司版权所有。
Objective: Analysis of the plasma N-glycome in endometriosis patients compared with controls.Study design: In a case-control study, blood samples were collected from patients who underwent either diagnostic or operative laparoscopy between 2008 and 2011 in the Semmelweis University, Budapest, I. Department of Obstetrics and Gynaecology. From these patients, 92 with endometriosis (30 stage I-II and 62 stage III-IV, including altogether 18 deep infiltrating cases) and 62 controls were selected for glycan analysis. After release, plasma N-glycans were subjected to hydrophilic interaction high performance liquid chromatography, which resulted in 19 chromatographic glycan peaks (GP). The abundances of the GPs were compared between the study groups. For statistical analysis a non-parametric test, the Mann-Whitney-U test, was used.Results: We found a statistically significant decrease of GP1 and increase of GP14, GP17 and GP18 in endometriosis patients. The latter peaks consist of glycans which play a role in inflammatory processes and malignancy. We also found significant differences in GP2, GP4, GP6, and GP9 between controls and the different endometriosis stage groups. The observed alterations in GP2, GP4 and GP6 may be related to altered glycosylation and remodelling of the glycan branches of the IgG molecule. The alterations of GP9 are presumably associated with changes of transferrin glycosylation. Furthermore we detected a highly significant decrease of GP1 in patients with deep infiltrating endometriosis compared with controls.Conclusions: This is the first analysis of the plasma N-glycome in endometriosis. The observed changes in GP14, GP17 and GP18 and in GP2, GP4, GP6 and GP9 provide new aspects to the pathophysiology of the disease and the alterations of the GP1 may serve as a new potential marker in the future. (C) 2013 Elsevier Ireland Ltd. All rights reserved.