Characterization and annotation of Babesia orientalis apicoplast genome.
Characterization and annotation of Babesia orientalis apicoplast genome.
复制标题
东方巴贝虫顶端质体基因组的表征和注释
DOI：
10.1186/s13071-015-1158-x
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发表时间：
2015-10-16
影响因子：
3.2
通讯作者：
Zhao J
中科院分区：
文献类型：
--
作者：
Huang Y;He L;Hu J;He P;He J;Yu L;Malobi N;Zhou Y;Shen B;Zhao J
东方巴氏线虫是布法罗红细胞内的一种专性寄生原虫，由蜱类扇头蜱传播。它是布法罗巴贝斯虫病的病原体，是中国中南部地区布法罗的重要病原之一。B. orientalis具有一个相对独立的、起源于植物的细胞器--顶质体。其他顶复门寄生虫中的顶质体参与血红素、脂肪酸、铁硫簇和类异戊二烯的生物合成。方法：根据B的全基因组序列设计30对引物。orientalis（未发表的数据）和比对已报道的牛巴伯虫和小泰勒虫的顶质体基因组。进行常规PCR以获得覆盖整个顶质体基因组的重叠片段。然后对东方B.orientalis的顶质体基因组进行测序、组装并与已报道的B的顶质体基因组进行比对。bovis和T. parva。利用Artemis软件对获得的顶质体基因组进行了注释，并与已发表的顶复门顶质体基因组进行了比较。将获得的SSU和LSU核苷酸序列用法师6.0软件进行系统发育分析。结果：获得了B的全基因组序列。东方顶质体它由33.2kb的环状DNA（78.9%A + T）组成.顶质体基因组单向编码一个大亚基和一个小亚基核糖体RNA、24个tRNA基因、4个DNA依赖性RNA聚合酶β亚基（rpoB、rpoC 1、rpoC 2a和rpoC 2b）、17个核糖体蛋白、1个EF-Tu延伸因子、2个Clp蛋白酶伴侣和14个假设蛋白。此外，它还包含两个clpC基因拷贝。B的结构和组织。orientalis顶质体基因组与B最为相似。结论：本研究首次报道了牛顶质体B基因的全序列。orientalis顶质体基因组。这些信息将有助于开发安全有效的治疗布法罗巴贝虫病的方法。
Background:Babesia orientalis is an obligate intraerythrocytic protozoan parasite of the buffalo (Bubalus bubalis, Linnaeus, 1758) transmitted by the tick Rhipicephalus heamaphysaloides. It is the causative agent of water buffalo babesiosis, one of the most important pathogens of water buffalo in central and southern China. As a member of the phylum Apicomplexa, B. orientalis possesses a relatively independent and alga originated organelle the apicoplast. Apicoplasts in other apicomplexa parasites are involved in the biosynthesis of haem, fatty acids, iron-sulphur clusters and isoprenoids. Some of these metabolic pathways were shown to be essential for parasite survival, therefore can serve as potential drug targets.Methods:30 pairs of primers were designed based on the full genome sequence of B. orientalis (unpublished data) and by aligning reported apicoplast genomes of Babesia bovis and Theileria parva. Conventional PCRs was performed to obtain overlapped fragments to cover the whole apicoplast genome. Then the apicoplast genome of B.orientalis was sequenced, assembled and aligned with reported apicoplast genomes of B. bovis and T. parva. The obtained apicoplast genome was annotated by using Artemis and comparing with published apicomplexan apicoplast genomes. The SSU and LSU nucleotide sequences generated were used in a phylogenetic analysis using the maximum likelihood implemented in MAGE 6.0.Results:We have obtained and analyzed the complete genome sequence of the B. orientalis apicoplast. It consisted of a 33.2 kb circular DNA (78.9 % A + T). The apicoplast genome unidirectionally encodes one large and one small subunit ribosomal RNAs, 24 tRNA genes, 4 DNA-dependent RNA polymerase beta subunits (rpoB, rpoC1, rpoC2a and rpoC2b), 17 ribosomal proteins, one EF-Tu elongation factor, 2 Clp protease chaperones, and 14 hypothetical proteins. In addition, it includes two copies of the clpC gene. The structure and organization of the B. orientalis apicoplast genome are most similar to those of the B. bovis apicoplast.Conclusions:This is the first report of the complete sequence of the B. orientalis apicoplast genome. This information should be useful in the development of safe and efficient treatment against buffalo babesiosis.