Quantification of proteins and metabolites by mass spectrometry without isotopic labeling or spiked standards
Quantification of proteins and metabolites by mass spectrometry without isotopic labeling or spiked standards
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DOI：
10.1021/ac026468x
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发表时间：
2003-09-15
影响因子：
7.4
通讯作者：
Becker, CH
中科院分区：
文献类型：
--
作者：
Wang, WX;Zhou, HH;Becker, CH
提出了一种新的方法，用于定量蛋白质组学和代谢组学的数据，液相色谱-质谱（LC-MS）与电喷雾电离。这种生物技术提供了差异表达测量，并能够发现生物标志物（生物标志物）。这里介绍的工作使用人血清，但适用于任何液体或组织。该方法依赖于信号与分子浓度的线性关系和样品处理的再现性。不使用同位素标记或化学相似的标准物质。报告了引入人血清的各种化合物的线性标准曲线。作为蛋白质组和代谢组取样分析重现性的测量，从25个独立处理的人血清样品中测定了类似于3400个分子离子（不计算其众多同位素）的中位变异系数分别为25.7%和23.8%，对应于总共85000个单个分子离子测量。
A new method is presented for quantifying proteomic and metabolomic profile data by liquid chromatography-mass spectrometry (LC-MS) with electrospray ionization. This biotechnology provides differential expression measurements and enables the discovery of biological markers (biomarkers). Work presented here uses human serum but is applicable to any fluid or tissue. The approach relies on linearity of signal versus molecular concentration and reproducibility of sample processing. There is no use of isotopic labeling or chemically similar standard materials. Linear standard curves are reported for a variety of compounds introduced into human serum. As a measure of analytical reproducibility for proteome and metabolome sampling, median coefficients of variation of 25.7 and 23.8%, respectively, were determined for similar to3400 molecular ions (not counting their numerous isotopes) from 25 independently processed human serum samples, corresponding to a total of 85 000 individual molecular ion measurements.