Associations between single nucleotide polymorphisms of MMP2, VEGF, and HIF1A genes and the risk of developing colorectal cancer.
Associations between single nucleotide polymorphisms of MMP2, VEGF, and HIF1A genes and the risk of developing colorectal cancer.
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发表时间：
2011-02
影响因子：
2
通讯作者：
Min-Jung Kang;S. Jung;J. Jung;Seong-Eun Kim;H. Jung;Tae‐Hun Kim;K. Shim;S. Yi;K. Yoo;
中科院分区：
文献类型：
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作者：
Min-Jung Kang;S. Jung;J. Jung;Seong-Eun Kim;H. Jung;Tae‐Hun Kim;K. Shim;S. Yi;K. Yoo;
本研究旨在探讨基质金属蛋白酶2（MMP 2）-1306 C/T、血管内皮生长因子（VEGF）936 C/T和缺氧诱导因子-1 α（HIF 1A）1772 C/T单核苷酸多态性（SNP）与结直肠癌风险之间的关系。患者和方法共50例结直肠癌患者（46%为女性，平均年龄68 ± 11岁）入组。没有癌症史或家族癌症易感性证据的健康对照组按性别和年龄（±5岁）与病例频率匹配。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法进行基因分型，分析基因型分布和风险估计。研究结直肠癌患者基因型与临床病理参数（Dukes分期、表型、部位、分化和大小）之间的相关性。结果HIF 1A 1772 C/T多态性T等位基因基因型分布与结直肠癌的发生有显著相关性（OR=3.63，95%可信区间，CI = 1.08-12.18，P = 0.03）。此外，当按年龄分层时，60岁以上患者中的相关性仍然存在（OR = 13.60，95% CI = 1.63-113.24，p = 0.01）。MMP 2、VEGF和HIF 1A基因型与结直肠癌的临床病理参数无相关性。结论HIF 1A 1772 C/T SNP与结直肠癌的发病风险显著相关，尤其是在60岁以上人群中。
UNLABELLED The aim of this study was to investigate the association between the risk for colorectal cancer and single nucleotide polymorphisms (SNP) of matrix metalloproteinase-2 (MMP2) -1306C/T, vascular endothelial growth factor (VEGF) 936C/T and hypoxia inducible factor-1α (HIF1A) 1772C/T. PATIENTS AND METHODS A total 50 colorectal cancer patients (46% women, mean age 68 ± 11 years) were enrolled. Healthy controls without evidence of cancer history or family cancer predispositions were frequency-matched to the cases by sex and age (±5 years). Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and the genotype distribution and risk estimate were analyzed. The correlation between the genotypes and clinicopathological parameters (Dukes stage, phenotype, location, differentiation and size) among colorectal cancer patients were investigated. RESULTS There was a significant association between colorectal cancer and T allele-bearing genotype distribution of HIF1A 1772C/T polymorphism (Odds ratio, OR=3.63, 95% confidence interval, CI = 1.08-12.18, p = 0.03 for CT and TT genotypes relative to CC genotype). In addition, when stratified by age, the association remained in patients older than 60 years old (OR = 13.60, 95% CI = 1.63-113.24, p = 0.01). However, there was no association between the genotypes of the MMP2, VEGF and HIF1A SNP and clinicopathological parameters of colorectal cancer. CONCLUSION There is a significant association between the HIF1A 1772C/T SNP and the risk of developing colorectal cancer, especially in individuals older than 60 years.