Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice.
Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice.
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2 型糖尿病 db/db 小鼠肾脏的转录谱
DOI：
10.1155/2017/8391253
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发表时间：
2017
影响因子：
4.3
通讯作者：
Li P
中科院分区：
文献类型：
--
作者：
Zhang H;Zhao T;Li Z;Yan M;Zhao H;Zhu B;Li P
糖尿病肾病是一种常见的糖尿病微血管并发症，以进行性肾小球硬化和肾小管间质纤维化为特征。然而，糖尿病肾病的潜在机制仍有待阐明。我们利用基因芯片技术研究了自发性2型糖尿病db/db小鼠转录水平的变化。与对照组db/m小鼠相比，db/db小鼠的体重、肾脏重量和尿白蛋白排泄量显著增加。肾脏组织学分析显示db/db小鼠肾脏系膜扩张，基底膜增厚。微阵列分析共鉴定出355个差异表达基因(DEG)。途径富集分析表明，生物氧化、胆汁酸代谢和类固醇激素合成是3条重要途径。前10个Hub基因是从构建的DGs的PPI网络中挑选出来的，包括Ccnb2和Nr1i2，它们在糖尿病肾病中仍然很大程度上不清楚。我们相信我们的研究有助于阐明糖尿病肾病的分子机制。
Diabetic nephropathy (DN), a common diabetic microvascular complication, is characterized by progressive glomerular sclerosis and tubulointerstitial fibrosis. However, the underlying mechanisms involved in DN remain to be elucidated. We explored changes in the transcriptional profile in spontaneous type 2 diabetic db/db mice by using the cDNA microarray. Compared with control db/m mice, the db/db mice exhibited marked increases in body weight, kidney weight, and urinary albumin excretion. Renal histological analysis revealed mesangial expansion and thickness of the basement membrane in the kidney of the db/db mice. A total of 355 differentially expressed genes (DEGs) were identified by microarray analysis. Pathway enrichment analysis suggested that biological oxidation, bile acid metabolism, and steroid hormone synthesis were the 3 major significant pathways. The top 10 hub genes were selected from the constructed PPI network of DEGs, including Ccnb2 and Nr1i2, which remained largely unclear in DN. We believe that our study can help elucidate the molecular mechanisms underlying DN.