Anti-inflammatory effect of stromal vascular fraction cells in fat transplantation
Anti-inflammatory effect of stromal vascular fraction cells in fat transplantation
复制标题
DOI：
10.3892/etm.2018.7082
复制
发表时间：
2019-02-01
影响因子：
2.7
通讯作者：
Liao, Qingting
中科院分区：
文献类型：
--
作者：
Zhu, Ming;Xue, Jian;Liao, Qingting
在以往的研究中，已经证明基质血管成分（SVF）通过其血管生成特性促进脂肪移植后移植物的留存率。然而，SVF对脂肪移植后炎症反应的影响尚不清楚。将来自C57小鼠的脂肪与绿色荧光蛋白（GFP）SVF细胞或磷酸盐缓冲盐水混合，并移植到C57 BL/6小鼠中。分别于术后1、7、14、30 d取材行免疫荧光染色。观察两组血管数、巨噬细胞数及炎症程度。虽然GFP荧光显示移植后大多数SVF细胞死亡，但与对照组相比，SVF辅助组的移植物留存率更高。与对照组相比，SVF辅助组还表现出更高的白细胞介素（IL）-6和肿瘤坏死因子-α表达水平，以及更低的IL-10水平，以及增加的M2巨噬细胞。因此，结果表明，SVF通过众所周知的促血管生成机制（旁分泌功能和参与新血管的形成）以及通过SVF的抗炎性质（表达和抑制各种细胞因子以及转化巨噬细胞表型）促进脂肪移植后移植物的留存率。
In previous studies, it has been demonstrated that the stromal vascular fraction (SVF) promoted the retention rate of grafts following fat transplantation through its angiogenic properties. However, the effect of SVF on inflammatory response subsequent to fat transplantation remains unclear. Fat from C57 mice were mixed with green fluorescent protein (GFP) SVF cells or phosphate-buffered saline and transplanted into C57BL/6 mice. The graft was harvested and immunofluorescent staining was performed on 1, 7, 14 and 30 days. The numbers of vessels and macrophages, as well as the inflammation level, in the two groups were evaluated. Although the GFP fluorescence indicated that most SVF cells were dead following transplantation, there was a higher graft retention rate in the SVF-assisted group compared with the control group. The SVF-assisted group also presented higher expression levels of interleukin (IL)-6 and tumor necrosis factor-, and lower levels of IL-10, as well as increased M2 macrophages in comparison with the control group. Therefore, the results indicated that SVF promoted the retention rate of grafts following fat transplantation through the well-known pro-angiogenic mechanism (paracrine function and involvement in the formation of new vessels), as well as through the anti-inflammatory property of SVF (expression and suppression of various cytokines and conversion of the macrophage phenotype).