Glass-coated, analyte-tagged nanoparticles: A new tagging system based on detection with surface-enhanced Raman scattering
Glass-coated, analyte-tagged nanoparticles: A new tagging system based on detection with surface-enhanced Raman scattering
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DOI：
10.1021/la026706j
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发表时间：
2003-05-27
期刊：
影响因子：
3.9
通讯作者：
Natan, MJ
中科院分区：
文献类型：
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作者：
Mulvaney, SP;Musick, MD;Natan, MJ
玻璃包覆的、带有分析物标记的纳米粒子（GANs）是核 - 壳结构的粒子，其中纳米级的金或银核被拉曼活性分子功能化，并包裹在玻璃壳中。玻璃壳为粒子提供了机械和化学稳定性。具体而言，玻璃包覆使得粒子能够在许多溶剂中使用而不改变拉曼光谱响应，并且不会发生团聚。玻璃壳的密度和厚度可通过合成条件控制；因此，可以调节通过二氧化硅网络的扩散速率，并使金属核与任何外部反应隔绝。这将允许生物分子附着在玻璃壳上而不改变拉曼响应。GANs可以通过所附着的拉曼标记的拉曼光谱来识别，并且两种不同标记的样品可以明确区分。拉曼标记的散射通过表面增强拉曼散射被放大。拉曼峰较窄的带宽（约20 cm⁻¹）和类似指纹的光谱将允许使用单一激发源同时评估多个拉曼标记。
Glass-coated, analyte-tagged nanoparticles (GANs) are core-shell particles where a nanometer-scale Au or Ag core is functionalized with Raman active molecules and encapsulated in a glass shell. The glass shell provides the particle with mechanical and chemical stability. Specifically, the glass coating renders the particle amenable to use in many solvents without altering the Raman spectral response and makes agglomeration a nonfactor. The density and thickness of the glass shell are controllable through synthetic conditions; thus, the rate of diffusion through the silica network can be tuned and the metal cores kept sequestered from any exterior reaction. This will allow for the attachment of biomolecules to the glass shell without altering the Raman response. GANs can be identified by the Raman spectrum of the attached Raman tag, and two differently labeled samples are unambiguously identified. The scattering from the Raman tag is amplified through surface-enhanced Raman scattering. The narrow bandwidth (similar to20 cm(-1)) of the Raman peaks and fingerprint-like spectra will allow multiple Raman tags to be simultaneously evaluated with a single excitation source.