Continuous, graded steady-state muscle work in rats studied by in vivo 31P-NMR.
Continuous, graded steady-state muscle work in rats studied by in vivo 31P-NMR.
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通过体内 31P-NMR 研究大鼠连续、分级的稳态肌肉工作。
DOI：
10.1152/jappl.1987.63.4.1428
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Chance,B
中科院分区：
文献类型：
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作者：
Argov,Z;Maris,J;Damico,L;Koruda,M;Roth,Z;LeighJr,JS;Chance,B
对运动人体肌肉的31P核磁共振波谱研究的理论考虑和实验结果表明，分级的、稳定状态的工作方案非常适合于健康和疾病方面的性能评估。我们描述了一个类似的重复31P-核磁共振研究的大鼠模型，该模型遵循了在正常人类对照组中观察到的许多31P-核磁共振特征。对水合氯醛麻醉的大鼠小腿肌肉进行4种频率(0.25、0.5、1.0和2.0 Hz)的间接刺激。结果表明：(1)在这个模型中可以短暂地维持几个稳定状态：(2)工作引起的磷酸肌酸(PCr)下降和无机磷(PI)上升是化学计量的：(3)刺激率与PI/PCr呈线性关系，斜率为2.01+/-0.4(+/-2SD，n=15)；4)未观察到ATP的显著下降，从而可以估计在此肌肉工作范围内的磷酸化电位(PP)的变化(静息状态下的PP为61,603+/-25,100M-1，运动结束时降至6,700+/-900M-1)；5)刺激后恢复较快，为2.27+/-0.5Pcr/PI U/min。这个简单的模型可以用于慢性动物肌肉疾病的长期研究。
Theoretical consideration and experimental findings of 31P nuclear magnetic resonance spectroscopy (NMR) studies of exercising human muscle suggest that a graded, steady-state work protocol is highly suitable for performance evaluation in health and disease. We describe a similar rat model for repeated 31P-NMR studies that follows many of the 31P-NMR features observed in normal human controls. Calf muscles of rats anesthetized with chloral hydrate were indirectly stimulated at four frequencies (0.25, 0.5, 1.0, and 2.0 Hz). It was found that 1) several steady states can be briefly maintained in this model; 2) work-induced phosphocreatine (PCr) fall and inorganic phosphate (Pi) rise is stoichiometric; 3) a linear relationship between stimulation rate and Pi/PCr was obtained, with a slope of 2.01 +/- 0.4 (+/- 2SD, n = 15); 4) no significant drop in ATP was observed, allowing the estimation of phosphorylation potential (PP) changes during this range of muscle work (PP at rest was 61,603 +/- 25,100 M-1 and fell to 6,700 +/- 900 M-1 at the end of exercise); and 5) poststimulation recovery was rapid, with a rate of 2.27 +/- 0.5 PCr/Pi U/min. This simple model can be used for prolonged studies of chronic animal muscle disorders.