Treatment with genetically engineered fibroblasts producing NGF or BDNF can accelerate recovery from traumatic spinal cord injury in the adult rat
Treatment with genetically engineered fibroblasts producing NGF or BDNF can accelerate recovery from traumatic spinal cord injury in the adult rat
复制标题
DOI：
10.1097/00001756-199609020-00033
复制
发表时间：
1996-09-02
期刊：
影响因子：
1.7
通讯作者：
Nockels, RP
中科院分区：
文献类型：
--
作者：
Kim, DH;Gutin, PH;Nockels, RP
我们验证了NGF或BDNF可以保护脊髓损伤后受损的神经结构的假设。用重物下落法造成成年大鼠脊髓挫伤。此后，将未修饰的大鼠1成纤维细胞或经工程改造以分泌NGF或BDNF的成纤维细胞注射到损伤部位。使用运动评定量表每周评估恢复情况，持续6周。所有大鼠立即截瘫，然后开始恢复。损伤后1周，植入NGF或BDNF分泌的成纤维细胞的大鼠的运动性能的评级显着增加与未修饰的成纤维细胞植入的大鼠。在实验期间，这种恢复增强的趋势持续存在，尽管差异变小了。6周后的组织学检查显示，用NGF或BDNF治疗的动物在最大损伤部位的脊髓横截面积更大。这些结果证明了在创伤性脊髓损伤中用神经营养素治疗的显著生物学效应。
WE tested the hypothesis that NGF or BDNF can protect damaged neural structures following spinal cord injury. Spinal contusions were produced in adult rats by a weight drop method. Thereafter, unmodified Rat 1 fibroblasts or fibroblasts engineered to secrete NGF or BDNF were injected into the injury site. Weekly assessments of recovery were made for 6 weeks using a locomotor rating scale. All rats were immediately paraplegic, then began to recover. At 1 week after injury, the ratings of locomotor performance in rats implanted with NGF- or BDNF-secreting fibroblasts were significantly increased over those of rats implanted with unmodified fibroblasts. This trend toward enhanced recovery persisted during the duration of the experiment, although the difference became smaller. Histological examination after 6 weeks showed a larger cross-sectional area of spinal cord at the maximal injury site in the animals treated with NGF or BDNF. these results demonstrate a significant biological effect of treatment with neurotrophins in traumatic spinal cord injury.