Serum folate by two methods in pregnant women carrying fetuses with neural tube defects.
Serum folate by two methods in pregnant women carrying fetuses with neural tube defects.
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通过两种方法测定怀有神经管缺陷胎儿的孕妇的血清叶酸。
DOI：
10.1093/clinchem/48.7.1094
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发表时间：
2002
影响因子：
9.3
通讯作者：
A. Moron
中科院分区：
文献类型：
--
作者：
Gizele Thame;E. Guerra;A. Moron
神经管缺陷 (NTD) 是最常见的严重先天畸形，是由于妊娠初期（受孕后 24-28 天）神经管闭合失败所致。遗传 (4)(2)(3)、环境 (4) 和营养因素 (5)(6)(7)(8)(9) 可能与 NTD 的病因有关。
在营养缺乏中，怀孕期间母亲的叶酸状况被认为是 NTD 最重要的危险因素之一，因此建议孕妇从补充剂和强化食品中摄入叶酸（600 微克/天）（10）。
叶酸是新组织形成过程中多种酶促反应的辅助因子。母体红细胞叶酸 (RCF) 和血清叶酸浓度低与 NTD 的发生密切相关 (8)(9)，特别是母体 RCF 浓度已被证明与 NTD 的发生呈浓度依赖性相关 (5)。然而，一些病例对照研究并未显示怀有 NTD 胎儿的孕妇的平均叶酸浓度较低 (6)(7)(9)。
所引用研究之间的差异可能归因于叶酸测定方法的内在差异。目前，用于叶酸测定的主要分析方法是微生物测定法、放射测定法、化学发光测定法、高效液相色谱法和离子捕获测定法。
本研究的目的是比较 55 名怀有未受影响胎儿或胎儿的孕妇通过两次自动叶酸测试获得的血清叶酸结果......
Neural tube defects (NTDs) are the most common severe congenital malformations that occur as a result of failure of neural tube closure at the beginning of pregnancy (24–28 days after conception). Genetic (4)(2)(3), environmental (4), and nutritional factors (5)(6)(7)(8)(9) can be involved in the etiology of NTDs.
Among the nutritional deficiencies, maternal folate status during pregnancy is considered one of the most important risk factors for NTDs, leading to a recommended dietary folate allowance for pregnant women (600 μg/day) from supplements and fortified food (10).
Folate is a cofactor in several enzymatic reactions in the formation of new tissues. Low maternal red cell folate (RCF) and serum folate concentrations have been strongly associated with the occurrence of NTDs (8)(9), and maternal RCF concentrations in particular have been shown to correlate with the occurrence of NTDs in a concentration-dependent fashion (5). However, some case-control studies did not show lower mean folate concentrations in pregnant women carrying fetuses with NTDs (6)(7)(9).
The differences among the cited studies may have been attributable to intrinsic differences in the methods used for folate determination. Currently, the major analytical approaches used for folate determination are microbiological assays, radioassays, chemiluminescence assays, HPLC, and ion-capture assays.
The aim of the present study was to compare serum folate results obtained by two automated folate tests in 55 pregnant women carrying unaffected fetuses or fetuses …