Production of fructo-oligosaccharides by levansucrase from Bacillus subtilis C4
Production of fructo-oligosaccharides by levansucrase from Bacillus subtilis C4
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DOI：
10.1016/s0032-9592(96)00058-1
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发表时间：
1997-03-01
影响因子：
4.4
通讯作者：
Combes, D
中科院分区：
文献类型：
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作者：
Euzenat, O;Guibert, A;Combes, D
研究了枯草芽孢杆菌C4产生的左旋糖苷酶在不同温度和蔗糖浓度条件下以蔗糖为原料生产左旋糖苷的效果。这些实验在高蔗糖浓度的溶液中进行，证明了左旋蔗糖酶能够转化反应介质中几乎所有可用的底物。反应产物分析表明，该酶催化低聚果糖和低聚果糖的形成，聚合度均小于50。用凝胶渗透法和阴离子交换高效液相色谱法对低聚果糖进行分析。随着反应介质中温度和蔗糖浓度的升高，链长减小。Levans和果寡糖同时形成。与菊粉和黑曲霉果糖转移酶产生的低聚果糖溶液的离子交换高效液相色谱图的比较表明，1-酮糖可能是反应介质中的主要低聚糖，还产生了许多其他低聚果糖，这表明左旋蔗糖酶对蔗糖形成的键的专一性很差。版权所有(C)1997爱思唯尔科学有限公司。
Levansucrase from Bacillus subtilis C4 was applied to the production of levans from sucrose, using various temperatures and sucrose concentrations. These experiments, performed in high sucrose concentration solutions, demonstrated the ability of levansucrase to transform almost all the substrate available in the reaction medium. Analysis of the reaction products showed the enzyme catalysed the formation of both levan and fructo-oligosaccharides with a degree of polymerisation smaller than 50.Fructo-oligosaccharides were analysed using gel permeation and anion exchange HPLC. The chain length decreased while temperature and sucrose concentration in the reaction medium increased. Levans and fructo-oligosaccharides were formed simultaneously. Comparison of our product profiles with ion exchange HPLC chromatograms of inulin and of a solution of fructo-oligosaccharides produced by Aspergillus niger fructosyl-transferase showed that 1-kestose was probably the main oligosaccharide in the reaction medium, and that a great variety of other fructo-oligosaccharides were also produced, suggesting very poor specificity of levansucrase for the linkages it forms from sucrose. Copyright (C) 1997 Elsevier Science Ltd.