Paper Bioassay Based on Ceria Nanoparticles as Colorimetric Probes
Paper Bioassay Based on Ceria Nanoparticles as Colorimetric Probes
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DOI：
10.1021/ac200697y
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发表时间：
2011-06-01
影响因子：
7.4
通讯作者：
Andreescu, Silvana
中科院分区：
文献类型：
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作者：
Ornatska, Maryna;Sharpe, Erica;Andreescu, Silvana
我们报告的氧化还原纳米氧化铈作为比色探针在生物分析中的第一次使用。该方法是基于氧化铈纳米粒子的物理化学性质的变化，在这里用作显色指示剂，响应于分析物。我们表明，这些颗粒可以完全集成在一个基于纸张的生物测定。为了构建传感器，二氧化铈纳米颗粒和葡萄糖氧化酶共固定到滤纸上使用硅烷化过程。在葡萄糖存在下，酶促产生的过氧化氢以浓度依赖性方式诱导固定在生物活性感测纸上的二氧化铈纳米颗粒的视觉颜色变化，从白黄色到深橙子。线性范围可达100 mM，n = 11的二氧化铈试纸条的重现性为4.3%，检测限为0.5 mM葡萄糖。该测定是完全可逆的，可以重复使用至少10个连续测量循环，而不会显着损失活性。另一个独特的特点是它不需要外部试剂，因为所有的传感元件都固定在纸平台上。生物测定可在室温下储存至少79天，同时保持相同的分析性能。以人血清中葡萄糖的检测为例进行了分析应用。结果表明，这种类型的纳米粒子作为新的组件在强大的比色生物测定的发展潜力。
We report the first use of redox nanoparticles of cerium oxide as colorimetric probes in bioanalysis. The method is based on changes in the physicochemical properties of ceria nanoparticles, used here as chromogenic indicators, in response to the analyte. We show that these particles can be fully integrated in a paper-based bioassay. To construct the sensor, ceria nanoparticles and glucose oxidase were coimmobilized onto filter paper using a silanization procedure. In the presence of glucose, the enzymatically generated hydrogen peroxide induces a visual color change of the ceria nanoparticles immobilized onto the bioactive sensing paper, from white-yellowish to dark orange, in a concentration-dependent manner. A detection limit of 0.5 mM glucose with a linear range up to 100 mM and a reproducibility of 4.3% for n = 11 ceria paper strips were obtained. The assay is fully reversible and can be reused for at least 10 consecutive measurement cycles, without significant loss of activity. Another unique feature is that it does not require external reagents, as all the sensing components are fixed onto the paper platform. The bioassay can be stored for at least 79 days at room temperature while maintaining the same analytical performance. An example of analytical application was demonstrated for the detection of glucose in human serum. The results demonstrate the potential of this type of nanoparticles as novel components in the development of robust colorimetric bioassays.