Measurement of Lysophospholipid Transport Across the Membrane Using Escherichia coli Spheroplasts.
Measurement of Lysophospholipid Transport Across the Membrane Using Escherichia coli Spheroplasts.
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使用大肠杆菌原生质球测量溶血磷脂跨膜运输。
DOI：
10.1007/978-1-4939-9136-5_13
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Bogdanov,Mikhail
中科院分区：
文献类型：
--
作者：
Lin,Yibin;Zheng,Lei;Bogdanov,Mikhail
在革兰氏阴性菌的内膜中，溶血磷脂转运体(LPLT)和双功能酰基载体蛋白(ACP)合成酶/2-酰基甘油磷乙醇胺酰基转移酶(AAS)形成甘油磷脂重塑系统，该系统能够促进溶血磷脂形式的磷脂乙醇胺、磷脂酰甘油和心磷脂快速逆行跨细胞膜转运。这种偶联的重塑酶序列为测定脂类再生和溶血磷脂本身跨膜转运的底物特异性提供了一种有效的方法。本章描述了两种截然不同但互为补充的方法，用于使用大肠杆菌原生质体测量溶血磷脂跨膜转运。
In the inner membrane of Gram-negative bacteria lysophospholipid transporter (LplT) and the bifunctional acyl-acyl carrier protein (ACP) synthetase/2-acylglycerolphosphoethanolamine acyltransferase (Aas) form a glycerophospholipid remodeling system, which is capable of facilitating rapid retrograde translocation of lyso forms of phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin across the cytoplasmic membrane. This coupled remodeling enzyme tandem provides an effective method for the measurement of substrate specificity of the lipid regeneration and lysophospholipid transport per se across the membrane. This chapter describes two distinct but complementary methods for the measurement of lysophospholipid transport across membrane usingEscherichia colispheroplasts.