Consequences of xenoestrogen exposure on male reproductive function in spottail shiners (Notropis hudsonius)
Consequences of xenoestrogen exposure on male reproductive function in spottail shiners (Notropis hudsonius)
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DOI：
10.1093/toxsci/kfh042
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发表时间：
2004-03-01
影响因子：
3.8
通讯作者：
Cyr, DG
中科院分区：
文献类型：
--
作者：
Aravindakshan, J;Paquet, V;Cyr, DG
关于在野外条件下与接触异雌激素有关的生理后果的资料有限。本研究的目的是确定圣劳伦斯河中雌激素化学物质的存在及其对斑点鱼（Notropis hudsonius）雄性生殖的影响。未成熟裸鼠肝脏卵黄蛋白原（VTG） mRNA水平表明，从蒙特利尔岛的上游和下游跨越近50公里的广泛雌激素污染。在不同程度的雌激素污染地点捕获的鱼进行了精子发生阶段的评估。在对照鱼中，95%有精子发生期IV期（50%）或V期（45%）的睾丸。在多尔瓦尔岛，VTG mRNA水平中等，鱼有III期睾丸（38%）和IV期睾丸（45%），只有15%的鱼处于生精期。相反，在Ilet Vert和Ile Beauregard，位于蒙特利尔市的污水流出羽流，鱼的肝脏VTG mRNA水平很高，没有鱼处于V期，Ilet Vert有8%的鱼处于II期发育。与来自Iles de la paaix的精子相比，来自Ilet Vert的精子浓度和各项运动参数显著降低（参考文献）。对睾丸的组织学分析显示，在雌激素污染最严重的地方捕获的鱼中，超过三分之一的鱼表现出双性，即卵巢卵泡在睾丸内发育的情况。这些数据表明，圣劳伦斯河中存在严重的雌激素污染，这与雄性鱼的生殖功能受损有关。
There is limited information on the physiological consequences associated with exposure to xenoestrogens under field conditions. The objectives of this study were to determine the presence of estrogenic chemicals in the St. Lawrence River and their effects on male reproduction in the spottail shiner (Notropis hudsonius). Hepatic vitellogenin (VTG) mRNA levels in immature shiners indicate extensive estrogenic contamination spanning almost 50 km both upstream and downstream from the island of Montreal. Stages of spermatogenesis were assessed in fish captured at sites having varying levels of estrogenic contamination. In control fish, 95% had testis of either stage IV (50%) or stage V (45%) of spermatogenesis. At Ile Dorval, where VTG mRNA levels are moderate, fish had testes of stage III (38%) and IV (45%) and only 15% of fish were at spermatogenic stage V. In contrast, at Ilet Vert and Ile Beauregard, located in the sewage effluent plume from the City of Montreal and where hepatic VTG mRNA levels are high in fish, none of the fish were at stage V and 8% of fish at Ilet Vert were at stage II of development. Sperm concentration and various motility parameters were significantly lower in shiners from Ilet Vert as compared with those from Iles de la Paix (reference). Histological analyses of testes revealed that more than one-third of the fish captured at sites with the highest estrogenic contamination displayed intersex, a condition in which ovarian follicles were developing within the testis. These data indicate that there is significant estrogenic contamination in the St. Lawrence River that is associated with impaired reproductive function in male fish.