Restricted fetal growth and lung development: A morphometric analysis of pulmonary structure
Restricted fetal growth and lung development: A morphometric analysis of pulmonary structure
复制标题
DOI：
10.1002/ppul.20480
复制
发表时间：
2006-12-01
影响因子：
3.1
通讯作者：
Owens, Julie A.
中科院分区：
文献类型：
--
作者：
Lipsett, Jill;Tamblyn, Michelle;Owens, Julie A.
人类宫内生长受限（IUGR）会增加肺部疾病和功能受损的风险，这表明不良的宫内状况会改变肺部发育。我们假设胎盘限制胎儿生长会改变妊娠晚期的肺结构。PR涉及移除妊娠前母羊的着床部位。在妊娠第140天分娩正常（n = 7）和PR（n = 11）胎儿。通过气管输注固定肺，通过形态计量学处理和分析。PR使羔羊的体重指数（PI）降低13%，增加肺体积：体重（BW）（19%），并使肺体积中包含实质的比例从86.5%（2.6%）降至76.7%（2.1%），而非实质的绝对体积无变化。在实质内，PR将包含空气空间的比例从42.0（2.2）%增加到55.5（1.7）%，存在较小（-13%）更密集（18%）的气囊/肺泡。总体效果是总气体交换表面密度降低（~ 10%）。所有动物的肺湿重和体积、实质体积、气体交换组织、气腔体积和气体交换表面积与BW和顶臀长度（CRL）呈正相关。相对肺重和肺体积与BW、CRL、肺重、BW与PI呈负相关。肺重量、肺体积、实质体积、气隙周长、实质气体交换组织百分比、气体交换表面密度和面积与PI呈正相关。结果表明，肺生长的保留增加，但结构变化增加，主要是在肺实质内，生长限制增加。与PR和胎儿生长不良相关的结构改变可能在与IUGR相关的肺功能受损的发病机制中起重要作用。
Intrauterine growth restriction (IUGR) in humans increases the risk of lung disease and impaired function suggesting that adverse intra-uterine conditions can alter lung development. We hypothesized that placental restriction (PR) of fetal growth would alter lung structure in late gestation. PR involved removal of implantation sites in pre-pregnant ewes. Normal (n = 7) and PR (n = 11) fetuses were delivered at day 140 gestation. Lungs were fixed by tracheal infusion, processed and analyzed by morphometry. PR reduced ponderal index (PI) of lambs by 13%, increased lung volume:body weight (BW) (19%), and decreased the proportion of lung volume that comprised parenchyma from 86.5(2.6)% to 76.7(2.1)% with no change in absolute volume of non-parenchyma. Within the parenchyma, PR increased the proportion comprising airspace from 42.0(2.2)% to 55.5(1.7)% with smaller (-13%) more dense (18%) airsacs/alveoli present. The overall effect was a reduction in total gas-exchange surface density (-10%). Lung wet-weight and volume, parenchymal volume, gas-exchange tissue, and airspace volumes and gas-exchange surface area correlated positively with BW and crown-rump length (CRL) for all animals. The relative lung weight and volume correlated negatively with BW, CRL, and lung weight:BW with PI. Lung weight, lung volume, parenchymal volume, airspace perimeter, percent of parenchymal gas-exchange tissue, gas-exchange surface density, and area correlated positively with PI. The results indicate increased sparing of lung growth but with increasing structural changes, predominantly within lung parenchyma, with increasing growth restriction. Structural alterations associated with PR and poor fetal growth may be important in the pathogenesis of impaired lung function associated with IUGR.