Surface plasmon resonance imaging of biomolecular interactions on a grating-based sensor array.
Surface plasmon resonance imaging of biomolecular interactions on a grating-based sensor array.
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DOI：
10.1021/ac0519209
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发表时间：
2006-01
影响因子：
7.4
通讯作者：
B. Singh;A. Hillier
中科院分区：
文献类型：
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作者：
B. Singh;A. Hillier
研制了一种基于光栅衬底的表面等离子体共振传感器阵列，用于检测生物分子间的相互作用。该衬底由一种商用可记录光盘的湿化学处理制备的金栅组成。构建了一种定制的浮针微观察仪，用于将含有omega功能化直链硫醇的溶液输送到光栅表面，并产生具有不同暴露功能端基的传感器元件阵列。该阵列平台可用于研究无标记、灵敏和高通量的生物分子相互作用。为了说明该设备的性能，将测试蛋白质(牛血清白蛋白)暴露在传感器元件中，传感器元件包含一系列具有激活的羧酸、胺或羟基末端区域的官能化烷硫醇。在固定角度成像配置中监测等离子激元共振的局部变化。等离子激元图像清楚地区分蛋白质在不同表面的附着程度。椭圆偏振测量仪也证实了光栅上的分子结合事件。这种基于光栅的SPR成像平台代表了一种简单而稳健的方法，用于执行生物分子相互作用的无标记、高灵敏度和高通量检测。
A surface plasmon resonance sensor array based upon a grating substrate was developed for the detection of biomolecular interactions. The substrate consisted of a gold grating prepared by wet chemical treatment of a commercial recordable compact disk. A custom-built floating pin microspotter was constructed to deliver solutions containing omega-functionalized linear alkanethiols to the grating surface and produce an array of sensor elements with different exposed functional end groups. This array platform can be used to study biomolecular interactions in a label-free, sensitive, and high-throughput format. To illustrate the performance of this device, a test protein (bovine serum albumin) was exposed to sensor elements containing an array of functionalized alkanethiols possessing either activated carboxylic acid-, amine-, or hydroxyl-terminated regions. Local changes in plasmon resonance were monitored in a fixed-angle imaging configuration. Plasmon images clearly distinguish the degree of protein attachment at the various surfaces. The molecular binding events on the grating were also confirmed by ellipsometry. This grating-based SPR imaging platform represents a simple and robust method for performing label-free, high-sensitivity, and high-throughput detection of biomolecular interactions.