Human vascular smooth muscle in culture. Growth and ultrastructure.
Human vascular smooth muscle in culture. Growth and ultrastructure.
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发表时间：
1975-07
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通讯作者：
M. Gimbrone;R. Cotran
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作者：
M. Gimbrone;R. Cotran
内皮细胞和平滑肌细胞的混合原代培养物是从人脐带血管中获得的，经过长时间的胶原酶消化其管腔表面。然后选择性地分离血管平滑肌的形态均一群体并传代培养长达16周。在超微结构上，培养的细胞的特点是存在束肌丝与致密体类似的天然脐血管平滑肌。成熟的培养物形成了一种独特的地形组织，由平行的细胞带和交织的多层区域组成。沿着沿着的细胞表面形成的精细的细胞间附着。大量的细胞外物质积累，包括无定形物质，弹性纤维微纤维，和250- 300-A，模糊的带状原纤维。在较老的文化，局灶性增殖，细胞外物质分泌和细胞变性产生结节状突起有点类似动脉粥样硬化病变在体内。从这个容易获得的人源培养的内皮细胞和平滑肌可能为血管疾病的病理生理学研究提供有用的比较材料。
Mixed primary cultures of endothelial and smooth muscle cells were obtained from human umbilical cord vessels after prolonged collagenase digestion of their luminal surfaces. Morphologically homogeneous populations of vascular smooth muscle were then selectively isolated and subcultured for up to 16 weeks. Ultrastructurally, cultured cells were characterized by the presence of bundles of myofilaments with dense bodies similar to native umbilical vessel smooth muscle. Mature cultures developed a distinctive topographical organization consisting of bands of parallel cells and intertwined, multilayered areas. Elaborate intercellular attachments formed along contiguous cell surfaces. Large amounts of extracellular material accumulated, including amorphous substance, elastic fiber microfibrils, and 250- to 300-A,faintly-banded fibrils. In older cultures, focal proliferation, extracellular material secretion and cellular degeneration produced nodular protrusions somewhat resembling atherosclerotic lesions in vivo. Endothelium and smooth muscle cultured from this readily available human source may provide useful comparative material for pathophysiologic studies of vascular disease.