An environmentally sensitive fluorescent purine nucleoside that changes emission wavelength upon hybridization.
An environmentally sensitive fluorescent purine nucleoside that changes emission wavelength upon hybridization.
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DOI：
10.1039/c3cc42605j
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发表时间：
2013-05
影响因子：
4.9
通讯作者：
Yoshio Saito;Azusa Suzuki;Y. Okada;Yuki Yamasaka;N. Nemoto;I. Saito
中科院分区：
文献类型：
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作者：
Yoshio Saito;Azusa Suzuki;Y. Okada;Yuki Yamasaka;N. Nemoto;I. Saito
合成了C7-萘乙炔基化的8-氮杂-7-脱氮-2 '-脱氧鸟苷（na）G，并对其物理性质进行了研究。荧光核苷具有溶剂化荧光显色性（Δλ（fl）（max）= 67 nm）。含有（na）G的ODN探针仅与C形成稳定的碱基对，并通过其发射波长的明显变化来区分结构变化，如错配和缺失。
C7-naphthylethynylated 8-aza-7-deaza-2'-deoxyguanosine (na)G was synthesized and its photophysical properties were examined. The fluorescent nucleoside exhibited solvatofluorochromic properties (Δλ(fl)(max) = 67 nm). An ODN probe containing (na)G forms a stable base pair only with C and discriminates structural changes such as mismatches and deletions by a distinct change in its emission wavelength.