CIS-DICHLORODIAMMINEPLATINUM(II) HAS A BINDING-SPECIFICITY FOR ADJOINING GUANINE BASES
CIS-DICHLORODIAMMINEPLATINUM(II) HAS A BINDING-SPECIFICITY FOR ADJOINING GUANINE BASES
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DOI：
10.1016/s0020-1693(00)82267-7
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
KIDANI, Y
中科院分区：
文献类型：
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作者：
INAGAKI, K;KIDANI, Y
自互补脱氧核糖八核苷酸，[5'-d(GpGpTpCpGpApCpC)-3']2 和 [5'-d(CpGpGp- ApTpCpCpG)-3']2，与顺式 PtCl2(NH3)2 反应，反应混合物用核酸外切酶（蛇毒磷酸二酯酶和/或小牛脾脏）处理磷酸二酯酶）。 HPLC 半定量分析表明，相邻的鸟嘌呤碱基对cis-PtCl2(NH3)2 具有特异性结合亲和力。
Self complementary deoxyribooctanucleotides, [5′-d(GpGpTpCpGpApCpC)-3′]2and [5’-d(CpGpGp- ApTpCpCpG)-3’]2, were allowed to react withcis- PtCl2(NH3)2, and the reaction mixture was treated with exonuclease (snake venom phosphodiesterase and/or calf spleen phosphodiesterase). Semi-quantitative analysis by HPLC showed that the adjoining guanine bases have a specific binding affinity tocis-PtCl2(NH3)2.