Cloning of Full-Length Genomic DNA Encoding Human FcϵRI α-Chain and Its Transcriptional Regulation
Cloning of Full-Length Genomic DNA Encoding Human FcϵRI α-Chain and Its Transcriptional Regulation
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编码人 FcϵRI α 链的全长基因组 DNA 的克隆及其转录调控
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
C. Ra
中科院分区：
文献类型：
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作者：
C. Nishiyama;M. Hasegawa;M. Nishiyama;Kyoko Takahashi;T. Yokota;K. Okumura;C. Ra
在人Fc γ RI α链基因第一外显子上游18.4和12.6kb处发现两个新的外显子，命名为外显子1A和外显子2A。外显子1A和外显子2A之间的“第一内含子”中引入突变，降低了外显子1A上游启动子的转录，表明内含子中存在内含子调控元件。与此一致的是，电泳迁移率变动试验显示存在一种核因子，其结合FcεRI α链阳性细胞中的区域。
Abstract Two novel exons, named exon 1A and exon 2A, were found at 18.4 and 12.6 kb upstream from the exon known as the first exon of human FcϵRI α-chain gene. Transcription from the promoter present in the upstream of exon 1A was decreased by mutations introduced into the “first intron” between exon 1A and exon 2A, suggesting the presence of an intronic regulatory element in the intron. Consistent with this, electrophoretic mobility shift assay revealed the presence of a nuclear factor which bound the region in FcϵRI α-chain positive cells.
U937 细胞中 IgA-Fc 受体 (Fc alpha R) 与 Fc epsilon RI gamma 2 亚基的关联。
DOI：
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发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Pfefferkorn,LC;Yeaman,GR
通讯作者：
Yeaman,GR