Bioherbicidal activity of drimane sesquiterpenes from Drimys brasiliensis Miers roots
Bioherbicidal activity of drimane sesquiterpenes from Drimys brasiliensis Miers roots
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DOI：
10.1016/j.indcrop.2015.04.042
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发表时间：
2015-11-15
影响因子：
5.9
通讯作者：
Berlinck, R. G. S.
中科院分区：
文献类型：
--
作者：
Anese, S.;Jatoba, L. J.;Berlinck, R. G. S.
该属植物是单萜和倍半萜类化合物的来源，在植物-昆虫、植物-病原体和植物-植物相互作用中具有潜在的作用。本研究的目的是确定在Drimys brasiliensis Miers根的次生代谢产物作为天然除草剂的潜力，小麦胚芽鞘生物测定。还评估了最具活性的化合物对黄藤（Barbarea verrta（Mill.））的发芽和早期生长的影响，稗草Echinochloa crus-galli（L.）和大叶牵牛（Ipomoea grandifolia（Dammer）奥唐纳）。从毛蕊花的活性部位中分离得到4个补身烷型倍半萜。通过H-1和C-13 NMR分析，鉴定为蓼二醛、蓼二醛缩酮、dendocarbin L和（+）-fuegin。所有四种分离的化合物在测试的最高浓度（ImM）下对小麦胚芽鞘具有植物毒性。在低浓度下，蓼二醛表现出最高水平的活性，对所有评估的目标物种具有植物毒性作用，并表现出对根伸长比芽早期发育更大的抑制活性。在洋地黄根的细胞水平上观察到了这种抑制作用，在所有评估的浓度下，木质部细胞的大小均显着减少。蓼二醛的植物毒性作用证明了该化合物用作生物除草剂的潜力。(C)2015 Elsevier B. V.版权所有。
Drimys genus plants are known as source of mono- and sesquiterpenoids compounds with potential roles in plant-insect, plant-pathogen and plant-plant interactions. The aim of this study was to identify secondary metabolites in the Drimys brasiliensis Miers roots with the potential to act as natural herbicides, using wheat coleoptile bioassays. The effects of the most active compound were also assessed on germination and early growth in Barbarea verrta (Mill.), Echinochloa crus-galli (L.) and Ipomoea grandifolia (Dammer) O'Donnell. Four drimane sesquiterpenes were isolated from bioactive fractions of D. brasiliensis roots and identified as polygodial, polygodial acetal, dendocarbin L and (+)-fuegin by H-1 and C-13 NMR analysis. All four of the isolated compounds were phytotoxic to the wheat coleoptiles at the highest concentration tested (1 mM). Polygodial showed the highest levels of activity at low concentrations, with a phytotoxic effect on all of the target species evaluated and exhibiting greater inhibitory activity toward root elongation than shoot early development. This inhibitory effect was observed at the cellular level in L grandifolia roots, with a significant reduction in the size of metaxylem cells at all of the evaluated concentrations. The phytotoxic effect of polygodial demonstrates the potential of this compound to be used as a bioherbicide. (C) 2015 Elsevier B.V. All rights reserved.