Effects of cell swelling on fluid flow across alveolar epithelium.
Effects of cell swelling on fluid flow across alveolar epithelium.
复制标题
细胞肿胀对穿过肺泡上皮的液体流动的影响。
DOI：
10.1152/jappl.1984.56.1.72
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Crandall,ED
中科院分区：
文献类型：
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作者：
Schaeffer,JD;Kim,KJ;Crandall,ED
本文研究了低温、代谢抑制和低渗对牛蛙肺泡上皮细胞内水通量的影响。从双脊牛蛙（Rana catesbeiana）中分离肺并制备成囊。渗透压（0.1 M棉子糖）或流体静力压（6.3-6.6 Torr）压力梯度施加在整个组织上。这些梯度导致从肺泡到胸膜浴的水的体积流量。控制水通量和水力传导率估计从肺囊的重量损失率。随后，在以下条件之一下测定水通量和水力传导率：1）将浴溶液的温度降低至2 ℃; 2）将ImM 2，4-二硝基苯酚加入肺泡浴和胸膜浴中，或3）将两种浴都改为半等渗林格溶液。渗透压（Lpo）和静水压（Lph）梯度的对照导水率分别为3.65（+/- 0.94）× 10（-12）和2.14（+/- 0.63）× 10（-10）ml/dyn × s。在代谢抑制、低渗和降低温度的条件下，Lph分别降低77%、83%和92%，Lpo分别降低56%、34%和59%。这些结果是最一致的假设，即在我们的实验条件下，在水力传导率的递减是由于上皮细胞肿胀，也许是在细胞旁通路的特性的变化。
The effects of decreased temperature, metabolic inhibition, and hyposmolality on osmotically and hydrostatically driven water flux across bullfrog alveolar epithelium were studied. Lungs were isolated from double-pithed Rana catesbeiana and prepared as sacs. Either an osmotic (0.1 M raffinose) or hydrostatic (6.3–6.6 Torr) pressure gradient was imposed across the tissue. These gradients resulted in the volume flow of water from the alveolar to pleural bath. Control water flux and hydraulic conductivity were estimated from the rate of weight loss of the lung sac. Subsequently water flux and hydraulic conductivity were determined under one of the following conditions: 1) temperature of bathing solutions lowered to 2 degrees C; 2) 1 mM 2,4-dinitrophenol added to both alveolar and pleural baths, or 3) both baths changed to half-isosmotic Ringer solution. The control hydraulic conductivities for osmotic (Lpo) and hydrostatic (Lph) pressure gradients were 3.65(+/- 0.94) X 10(-12) and 2.14(+/- 0.63) X 10(-10) ml/dyn X s, respectively. Under conditions of metabolic inhibition, hyposmolality, and decreased temperature, Lph decreased by 77, 83, and 92%, and Lpo decreased by 56, 34, and 59%, respectively. These results are most consistent with the hypothesis that the decrements in hydraulic conductivity under our experimental conditions are due to epithelial cell swelling and perhaps to changes in the characteristics of the paracellular pathway.