The acquired pellicle: immunofluorescent demonstration of specific proteins.
The acquired pellicle: immunofluorescent demonstration of specific proteins.
复制标题
获得的薄膜：特定蛋白质的免疫荧光演示。
DOI：
10.1111/j.1600-0714.1973.tb01675.x
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
Frederick W. Kraus
中科院分区：
文献类型：
--
作者：
Dag Orstavik;Frederick W. Kraus
。无菌抛光牛牙釉质板，装在小亚克力圆柱体中，在口腔中保持2小时。所获得的沉积在牙釉质表面的膜泡在平板上原位或转移到显微镜载玻片后进行检查。间接免疫荧光原位染色检测出免疫球蛋白a和溶菌酶阳性。首选的技术需要从牙釉质中酸分离膜，转移到显微镜载玻片上，并在冷乙醇中固定。从组织化学上看，该膜以碱性为主。间接免疫荧光示踪结果表明，IgA和溶菌酶是细胞膜的常规成分，淀粉酶和IgG的检测一致性较差，白蛋白和纤维蛋白原的检测很少。
. Sterile polished slabs of bovine enamel, mounted in small acrylic cylinders, were held in the mouth for 2 hours. The acquired pellicles deposited on the enamel surfaces were examined in situ on the slabs or after transfer to microscope slides. Indirect immunofluorescent staining in situ afforded positive identification of IgA and lysozyme in pellicles. The preferred technique required acid separation of the pellicle from the enamel, transfer to a microscope slide, and fixation in cold ethanol. Histochemically, this pellicle appeared to he predominantly basic. Results of indirect immunofluorescent tracing indicated that IgA and lysozyme were regular constituents of the pellicle, amylase and IgG were less consistently detected, and albumin and fibrinogen were rarely encountered.